Wei Yuanyi, La Lei, Wang Lili, Batey Robert, Wang Chunxia, Li Yuhao
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Central Clinical School, Royal Prince Alfred Hospital, The University of Sydney, Australia.
J Ethnopharmacol. 2017 May 23;204:36-44. doi: 10.1016/j.jep.2017.03.054. Epub 2017 Apr 7.
Paeoniflorin and liquiritin are major constituents in some Chinese herbal formulas, such as Yiru Tiaojing (YRTJ) Granule (a hospitalized preparation) and Peony-Glycyrrhiza Decoction, used for hyperprolactinemia-associated disorders.
To investigate the effect of paeoniflorin and liquiritin on prolactin secretion.
The effect of YRTJ Granule on metoclopramide-induced hyperprolactinemia was tested in rats. Paeoniflorin and liquiritin in the YRTJ Granule extract were identified and quantified by HPLC. The effects of paeoniflorin and liquiritin on prolactin secretion were examined in prolactinoma cells that were identified morphologically and by Western blot. The concentration of prolactin was determined by ELISA. The gene expression was analyzed by Western blot.
YRTJ Granule ameliorated metoclopramide-induced hyperprolactinemia in rats. The contents of paeoniflorin and liquiritin in YRTJ Granule were 7.43 and 2.05mg/g extract, respectively. Paeoniflorin, liquiritin and bromocriptine (a dopamine D receptor (DR) agonist) decreased prolactin concentration in MMQ cells expressing DR. However, the effect of liquiritin and bromocriptine was abolished in GH3 cells lacking DR expression. Interestingly, paeoniflorin still decreased prolactin concentration in GH3 cells in the same manner. Furthermore, paeoniflorin suppressed prolactin protein expression, and was without effect on DR protein expression in both MMQ and GH3 cells.
The present results suggest that paeoniflorin and liquiritin play a role in YRTJ Granule-elicited improvement of hyperprolactinemia. While the effect of liquiritin is DR-dependent, paeoniflorin DR-independently inhibits prolactin secretion in prolactinoma cells that may especially benefit the hyperprolactinemic patients who are refractory to dopaminergic therapies.
芍药苷和甘草苷是一些中药方剂中的主要成分，如抑乳调经（YRTJ）颗粒（一种院内制剂）和芍药甘草汤，用于治疗与高催乳素血症相关的疾病。
研究芍药苷和甘草苷对催乳素分泌的影响。
在大鼠中测试YRTJ颗粒对胃复安诱导的高催乳素血症的作用。通过高效液相色谱法对YRTJ颗粒提取物中的芍药苷和甘草苷进行鉴定和定量。在通过形态学和蛋白质印迹鉴定的催乳素瘤细胞中检测芍药苷和甘草苷对催乳素分泌的影响。通过酶联免疫吸附测定法测定催乳素浓度。通过蛋白质印迹分析基因表达。
YRTJ颗粒改善了大鼠胃复安诱导的高催乳素血症。YRTJ颗粒中芍药苷和甘草苷的含量分别为7.43和2.05mg/g提取物。芍药苷、甘草苷和溴隐亭（一种多巴胺D受体（DR）激动剂）降低了表达DR的MMQ细胞中的催乳素浓度。然而，在缺乏DR表达的GH3细胞中，甘草苷和溴隐亭的作用消失。有趣的是，芍药苷仍以相同方式降低GH3细胞中的催乳素浓度。此外，芍药苷抑制催乳素蛋白表达，并且对MMQ和GH3细胞中的DR蛋白表达均无影响。
目前的结果表明，芍药苷和甘草苷在YRTJ颗粒引起的高催乳素血症改善中起作用。虽然甘草苷的作用依赖于DR，但芍药苷不依赖DR抑制催乳素瘤细胞中的催乳素分泌，这可能对多巴胺能治疗难治的高催乳素血症患者特别有益。
