通过结合测定和亲和交联分析KYM细胞上的TNF受体。
Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking.
作者信息
Niitsu Y, Watanabe N, Sone H, Neda H, Yamauchi N, Maeda M, Urushizaki I
机构信息
Department of Internal Medicine, Sapporo Medical College, Japan.
出版信息
J Biol Response Mod. 1988 Jun;7(3):276-82.
PMID:2839624
Abstract
The existence of a tumor necrosis factor (TNF) receptor on KYM human myosarcoma cells was elucidated by Scatchard plot and cross-linking analysis. The average number of specific binding sites per cell was 15,300 and the apparent dissociation constant Kd was 4.0 X 10(-10) M. The estimated relative molecular mass of a TNF receptor on KYM cell was approximately 95,000.
摘要
通过Scatchard图和交联分析阐明了KYM人肌肉肉瘤细胞上肿瘤坏死因子（TNF）受体的存在。每个细胞上特异性结合位点的平均数为15300个，表观解离常数Kd为4.0×10⁻¹⁰ M。KYM细胞上TNF受体的估计相对分子质量约为95000。
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