Sadoul B, Friggens N C, Valotaire C, Labbé L, Colson V, Prunet P, Leguen I
INRA, LPGP Fish Physiology and Genomics UR1037, Rennes, France.
INRA UMR 0791 Modélisation Systémique Appliquée aux Ruminants, INRA, AgroParisTech, Université Paris-Saclay, Paris 75005, France.
Comp Biochem Physiol A Mol Integr Physiol. 2017 Jul;209:25-33. doi: 10.1016/j.cbpa.2017.04.002. Epub 2017 Apr 7.
Adaptive capacities, governing the ability of animals to cope with an environmental stressor, have been demonstrated to be strongly dependent upon genetic factors. Two isogenic lines of rainbow trout, previously described for their sensitivity and resilience to an acute confinement challenge, were used in the present study to investigate whether adaptive capacities remain consistent when fish are exposed to a different type of challenge. For this purpose, the effects of a 4-hour hypercapnia (CO increase) challenge at concentrations relevant in aquaculture conditions are described for the two isogenic lines. Oxygen consumption, cortisol release, group dispersion and group swimming activity were measured before, during and after the challenge. Sensitivity and resilience for each measure were extracted from temporal responses and analyzed using multivariate statistics. The two fish lines displayed significant differences in their cortisol response, translating differences in the stress axis sensitivity to the stressor. On the contrary, both lines showed, for other measures, similar temporal patterns across the study. Notable within line variability in the stress response was observed, despite identical genome between fish. The results are discussed in the context of animal robustness.
适应能力决定动物应对环境应激源的能力，已被证明强烈依赖于遗传因素。本研究使用了两个先前因其对急性限制挑战的敏感性和恢复力而被描述的虹鳟同基因品系，来调查当鱼类面临不同类型的挑战时，其适应能力是否保持一致。为此，描述了在水产养殖条件下相关浓度的4小时高碳酸血症（二氧化碳增加）挑战对这两个同基因品系的影响。在挑战前、挑战期间和挑战后测量了氧气消耗、皮质醇释放、群体分散和群体游泳活动。从时间反应中提取每项测量的敏感性和恢复力，并使用多元统计进行分析。这两个鱼品系在皮质醇反应上表现出显著差异，这反映了应激轴对应激源的敏感性差异。相反，对于其他测量，两个品系在整个研究中都显示出相似的时间模式。尽管鱼之间的基因组相同，但在应激反应中观察到了品系内显著的变异性。结果在动物稳健性的背景下进行了讨论。
