Wang Jiaojiao, Zhou Rongyi, Gao Weiping
Nanjing University of Traditional Chinese Medicine, Xianlin road no. 138, Qixia District, Nanjing City, Jiangsu Province 210023, China.
Neurosci Lett. 2017 May 10;649:14-19. doi: 10.1016/j.neulet.2017.04.012. Epub 2017 Apr 7.
We investigated the neural pathway for tear secretion from the lacrimal gland of New Zealand White rabbits.
Nine healthy adult New Zealand White rabbits were randomly divided into three experimental groups, namely, an irritant-stimulated group, a non-stimulated group, and a saline-stimulated group. Sanitized dry cotton swabs with menthol were used to wipe both of the rabbits' eyelids in the irritant-stimulated group, and the non-stimulated group and saline- stimulated group were compared as controls. The animals in the three groups were killed 2h later and the expressions of c-Fos in the frontal cortex, hippocampus, hypothalamus, pons, and medulla oblongata of the rabbits were detected using immunofluorescence labeling. According to the distribution of c-Fos protein expression, 12 healthy adult New Zealand rabbits were similarly divided into three groups for retrograde tract tracing via pseudorabies virus (PRV) injection into the lacrimal gland. Immunofluorescence labeling was used to analyze PRV-infected neurons in the brains of rabbits after survival for 30h, 38h, and 46h.
The most c-Fos-positive immunolabeled cells were observed in the menthol-stimulated group, whereas fewer c-Fos-positive immunolabeled cells were observed in the saline-stimulated group.The non-treated group showed the least c-Fos-positive immunolabeled cells. At 30h after PRV injection, PRV-positive neurons were found only in the superior salivary nucleus of the pons (SSN). At 38h, PRV-infected neurons were observed in the lateral nucleus of the superior olive (LSO) and the medial nucleus of the superior olive (MSO). At 46h, PRV-infected neurons were found in the nucleus of the trapezoid body (Tz) and the hypothalamic paraventricular nucleus (PVN), and their distributions were dense in the LSO and MSO.
Menthol-induced c-Fos protein expression and PRV-mediated tract tracing suggest that in New Zealand White rabbits, the neural pathway that regulates tear secretion from the lacrimal gland proceeds from the PVN to the superior olivary complex of the pons to the SSN and finally to the lacrimal gland.
我们研究了新西兰白兔泪腺泪液分泌的神经通路。
将9只健康成年新西兰白兔随机分为三个实验组，即刺激组、非刺激组和生理盐水刺激组。刺激组用含薄荷醇的消毒干棉签擦拭兔子的双眼睑，非刺激组和生理盐水刺激组作为对照。2小时后处死三组动物，采用免疫荧光标记法检测兔子额叶皮质、海马、下丘脑、脑桥和延髓中c-Fos的表达。根据c-Fos蛋白表达分布，将12只健康成年新西兰白兔同样分为三组，通过向泪腺注射伪狂犬病病毒（PRV）进行逆行示踪。存活30小时、38小时和46小时后，用免疫荧光标记法分析兔子脑中PRV感染的神经元。
薄荷醇刺激组观察到的c-Fos阳性免疫标记细胞最多，而生理盐水刺激组观察到的c-Fos阳性免疫标记细胞较少。未处理组的c-Fos阳性免疫标记细胞最少。PRV注射后30小时，仅在脑桥的上涎核（SSN）发现PRV阳性神经元。38小时时，在上橄榄外侧核（LSO）和上橄榄内侧核（MSO）观察到PRV感染的神经元。46小时时，在梯形核（Tz）和下丘脑室旁核（PVN）发现PRV感染的神经元，且它们在LSO和MSO中的分布密集。
薄荷醇诱导的c-Fos蛋白表达和PRV介导的示踪表明，在新西兰白兔中，调节泪腺泪液分泌的神经通路从PVN出发，经脑桥的上橄榄复合体至SSN，最终到达泪腺。
