通过RNA干扰靶向乳腺癌耐药蛋白/三磷酸腺苷结合盒转运体G2可增强人结肠癌侧群细胞的化疗敏感性。
Targeting BCRP/ABCG2 by RNA interference enhances the chemotherapy sensitivity of human colon cancer side population cells.
作者信息
Hu Jun, Li Jian, Yue Xin, Wang Jia-Cang, Wang Jun-Feng, Liu Jian-Zhong, Kong Da-Lu
机构信息
Department of Colorectal Cancer Surgery, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China.
Department of Lymphoma, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
出版信息
J Huazhong Univ Sci Technolog Med Sci. 2017 Apr;37(2):231-236. doi: 10.1007/s11596-017-1720-1. Epub 2017 Apr 11.
Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics. This study isolated side population (SP) cells from a colon cancer cell line (Colo-320) and examined their self-renewal and differentiation abilities. Compared to non-SP (NSP) cells, SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies. Additionally, more cells were in G/G phase and more highly expressed the multidrug resistance protein BCRP/ABCG2. We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like, small interfering RNA (siRNA) eukaryotic expression plasmid targeting BCRP/ABCG2.
结肠癌中复发和转移很常见，可能与干细胞特性有关。本研究从结肠癌细胞系（Colo-320）中分离出侧群（SP）细胞，并检测了它们的自我更新和分化能力。与非SP（NSP）细胞相比，SP结肠癌细胞在体内具有更强的致瘤性，表现出更强的侵袭特性和更大的集落形成能力。此外，更多细胞处于G/G期，多药耐药蛋白BCRP/ABCG2的表达更高。我们通过用靶向BCRP/ABCG2的发夹状小干扰RNA（siRNA）真核表达质粒转染SP细胞，提高了化疗敏感性。
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