Lee W H, Yeh M Y, Tu Y C, Han S H, Wang Y C
Institute of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
J Surg Oncol. 1988 Jul;38(3):173-81. doi: 10.1002/jso.2930380309.
A permanent human brain malignant glioma cell line, GBM8401/TSGH,NDMC, has been successfully established from a 31-year-old Chinese female with brain glioblastoma multiforme (GBM) in monolayer culture and has been subcultured for more than 100 passages during 24 months in vitro. The tumor cell doubling time in vitro was approximately 38 hr. The tumorigenicity in athymic nude mice was observed; the tumor volume doubling time was approximately 4 days. Glial fibrillary acidic protein (GFAP) and 10-nm-diameter intermediate filaments were identified by immunohistochemical peroxidase-antiperoxidase (PAP), immunofluorescence assay, and transmission electron microscopic methods. The scanning electron microscope revealed numerous surface microvilli and various-sized blebs. Karyotypic analysis showed this malignant glioma cell line to be of human origin, near-diploid with a modal chromosome number of 48,XX.
已成功从一名31岁患有多形性胶质母细胞瘤（GBM）的中国女性患者中建立了一种永久性人脑恶性胶质瘤细胞系GBM8401/TSGH、NDMC，采用单层培养法，在体外24个月内已传代100多次。该肿瘤细胞在体外的倍增时间约为38小时。观察了其在无胸腺裸鼠中的致瘤性；肿瘤体积倍增时间约为4天。通过免疫组织化学过氧化物酶-抗过氧化物酶（PAP）、免疫荧光分析和透射电子显微镜方法鉴定了胶质纤维酸性蛋白（GFAP）和直径为10纳米的中间丝。扫描电子显微镜显示有大量表面微绒毛和各种大小的泡状突起。核型分析表明该恶性胶质瘤细胞系起源于人，接近二倍体，众数染色体数为48，XX。
