Hernández-Díaz Nathaly, Torres Rodrigo, Ramírez-Pinilla Martha Patricia
Laboratorio de Biología Reproductiva de Vertebrados, Escuela de Biología, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia.
Grupo de Investigación en Bioquímica y Microbiología, GIBIM, Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia.
J Exp Zool B Mol Dev Evol. 2017 Jun;328(4):371-389. doi: 10.1002/jez.b.22739. Epub 2017 Apr 11.
Reptiles are one of the most diverse groups of vertebrates, providing an integrated system for comparative studies on metabolic, animal physiology, and developmental biology. However, the molecular data available are limited and only recently have started to call attention in the "omics" sciences. Mabuya sp. is a viviparous placentrotrophic skink with particular reproductive features, including microlecithal eggs, early luteolysis, prolonged gestation, and development of a highly specialized placenta. This placenta is responsible for respiratory exchange and the transference of all nutrients necessary for embryonic development. Our aim was to identify differentially expressed proteins in the ovary and placenta of Mabuya sp. during early, mid, and late gestation; their possible metabolic pathways; and biological processes. We carried out a comparative proteomic analysis during gestation in both tissues by sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis, and matrix-assisted laser desorption/ionization. Differential protein expression in both tissues (Student's t-test P < 0.05) was related to several processes such as cell structure, cell movement, and energy. Proteins found in ovary are mainly associated with follicular development and its regulation. In the placenta, particularly during mid and late gestation, protein expression is involved in nutrient metabolism, transport, protein synthesis, and embryonic development. This work provides new insights about the proteins expressed and their physiological mechanisms in Mabuya sp. placenta and ovary during gestation.
爬行动物是脊椎动物中最多样化的类群之一，为代谢、动物生理学和发育生物学的比较研究提供了一个综合系统。然而，现有的分子数据有限，直到最近才开始在“组学”科学中引起关注。 Mabuya sp. 是一种胎生胎盘营养的石龙子，具有特殊的生殖特征，包括微卵黄卵、早期黄体溶解、妊娠期延长以及高度特化胎盘的发育。这种胎盘负责呼吸交换和胚胎发育所需的所有营养物质的转移。我们的目的是鉴定 Mabuya sp 在妊娠早期、中期和晚期卵巢和胎盘中差异表达的蛋白质；它们可能的代谢途径；以及生物学过程。我们通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳、二维凝胶电泳和基质辅助激光解吸/电离对两种组织在妊娠期进行了比较蛋白质组学分析。两种组织中的差异蛋白表达（学生t检验P < 0.05）与细胞结构、细胞运动和能量等几个过程有关。在卵巢中发现的蛋白质主要与卵泡发育及其调节有关。在胎盘中，特别是在妊娠中期和晚期，蛋白质表达参与营养物质代谢、运输、蛋白质合成和胚胎发育。这项工作为 Mabuya sp. 在妊娠期胎盘和卵巢中表达的蛋白质及其生理机制提供了新的见解。
