马立克氏病病毒DNA的结构：IRs和TRs中重复序列的扩增

The structure of Marek's disease virus DNA: amplification of repeat sequence in IRs and TRs.

作者信息

Hayashi M, Jessip J, Fukuchi K, Smith M, Tanaka A, Nonoyama M

机构信息

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo.

出版信息

Microbiol Immunol. 1988;32(3):265-74. doi: 10.1111/j.1348-0421.1988.tb01386.x.

DOI:10.1111/j.1348-0421.1988.tb01386.x

PMID:2839751

Abstract

The structure of Marek's disease virus (MDV) DNA was investigated by using Southern blot hybridization analysis. A heterogeneous region was observed in the inverted repeats region, IRs and TRs, as well as in the TRL and IRL. The results of DNA sequencing of the heterogeneous region showed that the heterogeneity of IRS and TRS was due to amplification of a 178-bp repeat sequence. Amplification of IRS and TRS was found in viral DNA from both pathogenic and nonpathogenic strains. The structure of DNA from the latent MDV genome present in established lymphoblastic cells was also determined. Amplification of the 132-bp repeat sequence in IRL and TRL was not found in latent MDV DNA of established lymphoblastic cells, whereas amplification of the 178-bp repeat sequence in IRS and TRS was found in the same DNA.

摘要

通过Southern印迹杂交分析研究了马立克氏病病毒（MDV）DNA的结构。在反向重复区域（IRs和TRs）以及TRL和IRL中观察到一个异质区域。对该异质区域的DNA测序结果表明，IRS和TRS的异质性是由于一个178bp重复序列的扩增。在致病和非致病菌株的病毒DNA中均发现了IRS和TRS的扩增。还确定了存在于已建立的淋巴细胞中的潜伏MDV基因组的DNA结构。在已建立的淋巴细胞的潜伏MDV DNA中未发现IRL和TRL中132bp重复序列的扩增，而在同一DNA中发现了IRS和TRS中178bp重复序列的扩增。