Nakano Takafumi
Department of Science Education, Graduate School of Education, Hiroshima University, Higashihiroshima, Hiroshima 739-8524, Japan.
Zoolog Sci. 2017 Apr;34(2):161-172. doi: 10.2108/zs160167.
A quadrannulate species of Orobdella, O. kanaekoikeae sp. nov., from the mountainous region of Shikoku, Japan is described. This new species is small with a body length of mature individuals reaching only ca. 5 cm. Phylogenetic analyses using nuclear 18S, histone H3, mitochondrial cytochrome c oxidase subunit I, tRNA, tRNA, 12S rRNA, tRNA, 16S rRNA, tRNA, and NADH dehydrogenase subunit 1 markers showed that O. kanaekoikeae is a sister species of the quadrannulate small O. brachyepididymis. Phylogenetic relationships among populations of this new species were also estimated using mitochondrial DNA markers. Additionally, transmission electron micrographs of a capsule-like structure obtained from a post-copulatory individual revealed that the capsule contained spermatozoa, and was thus defined as the spermatophore. The gastroporal duct of Orobdella leeches is an accessory copulatory organ that receives a spermatophore during copulation.
描述了一种来自日本四国山区的具四环带的蛭类物种，即金池蛭（Orobdella kanaekoikeae）。该新物种体型较小，成熟个体体长仅约5厘米。使用核18S、组蛋白H3、线粒体细胞色素c氧化酶亚基I、tRNA、tRNA、12S rRNA、tRNA、16S rRNA、tRNA和NADH脱氢酶亚基1标记进行的系统发育分析表明，金池蛭是具四环带的小型短附睾蛭的姐妹物种。还使用线粒体DNA标记估计了该新物种种群之间的系统发育关系。此外，从交配后个体获得的一种胶囊状结构的透射电子显微镜照片显示，该胶囊包含精子，因此被定义为精荚。蛭类的腹孔管是一种辅助交配器官，在交配时接收精荚。
