心房利钠多肽（ANP）受体在肾小球中的定位：使用125I标记的ANP进行体外电子显微镜放射自显影研究。

Localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus: in vitro electron microscopic autoradiographical investigation using 125I-labeled ANP.

作者信息

Mizukawa K, Ogura T, Yamamoto I, Mitsui T, Katayama E, Ota Z, Ogawa N

机构信息

Department of Anatomy, Okayama University Medical School, Japan.

出版信息

Regul Pept. 1988 May;21(1-2):167-72. doi: 10.1016/0167-0115(88)90100-0.

DOI:10.1016/0167-0115(88)90100-0

PMID:2839872

Abstract

The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus.

摘要

利用¹²⁵I标记的心房利钠多肽（ANP），通过电子显微镜放射自显影术研究了大鼠肾小球中ANP受体的定位。在30个肾小球中计数的总共1134个银颗粒分布如下：足细胞突起（36.4%）、足细胞胞体（14.8%）、基底膜（12.3%）、内皮细胞（6.3%）、系膜细胞（7.7%）以及其他部位，如血管间隙（22.5%）。这一发现表明ANP结合位点主要定位于肾小球中足细胞的足突上。