新生霉素可抑制猴空泡病毒40增强子活性。

Novobiocin inhibits the SV40 enhancer activity.

作者信息

Kohno K, Iwamoto Y, Martin G R, Yamada Y

机构信息

Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, Maryland 20892.

出版信息

Biochem Biophys Res Commun. 1988 Jul 15;154(1):483-8. doi: 10.1016/0006-291x(88)90712-7.

DOI:10.1016/0006-291x(88)90712-7

PMID:2840073

Abstract

Using a transient expression assay, we have analysed the effect of novobiocin, DNA topoisomerase II inhibitor, on simian virus 40(SV40) enhancer activities. We used the recombinant clones containing type I or II collagen promoters placed upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene with or without SV40 enhancer. We observed the expected increase in CAT activities due to the presence of the SV40 enhancer. Interestingly, CAT gene expression of the enhancer-containing constructs were inhibited more sensitively by novobiocin than that of the enhancer-less construct. This findings lead us propose that DNA superhelicity mediated by topoisomeraseII is one of the important factor for the manifestation of SV40 enhancer activity.

摘要

利用瞬时表达分析，我们分析了新生霉素（一种DNA拓扑异构酶II抑制剂）对猿猴病毒40（SV40）增强子活性的影响。我们使用了重组克隆，这些克隆包含位于细菌氯霉素乙酰转移酶（CAT）基因上游的I型或II型胶原启动子，有或没有SV40增强子。由于SV40增强子的存在，我们观察到CAT活性如预期那样增加。有趣的是，与不含增强子的构建体相比，含增强子构建体的CAT基因表达受到新生霉素的抑制更敏感。这些发现使我们提出，由拓扑异构酶II介导的DNA超螺旋是SV40增强子活性表现的重要因素之一。