Li Ting, Ma Yuanyuan, Zhang Hong, Yan Ping, Huo Lili, Hu Yongyan, Chen Xi, Li Ting, Zhang Miao, Liu Zhaohui
Department of Obstetrics and Gynecology, Peking University First Hospital, 8 Xishiku Street, Beijing 100034, China.
Animal Center Laboratory, Peking University First Hospital, 8 Xishiku Street, Beijing 100034, China.
Biomed Res Int. 2017;2017:7456853. doi: 10.1155/2017/7456853. Epub 2017 Mar 16.
. Our previous Gräfenberg spot findings confirmed that the distal-third areas of the anterior vaginal wall bore a significantly greater number of nerves and sexual hormone may have certain degree of influence on these significant differences. However, the role of estrogen in vaginal innervations remains controversial. . To investigate whether hormonal-neural interactions occur in the vagina, sixty rats were randomly divided into six groups: Sham-operated, ovariectomy, and 4 treatment groups. After 2 weeks of treatment, vaginal biopsies were prepared with hematoxylin and eosin and PGP9.5 using immunohistochemistry. . The density of small nerve fibers was significantly higher in the distal-half areas of intact vaginal walls than the proximal-half areas ( = 0.001). In contrast, the overall PGP 9.5-ir fiber innervation density was significantly decreased in the OVX rats subjected to surgical menopause. Sustained estrogen administration for 2 weeks resulted in nerve fiber proliferation, with values reaching normal levels in the low-dose estradiol valerate group. . Our findings indicate that systemic hormonal therapy with low-dose estradiol valerate is effective and safe for treating deficient vaginal innervation caused by low level of estrogen activity in menopausal women and may aid studies to identify an optimal estradiol dose to provide relief from vaginal discomfort.
我们之前关于格拉芬伯格点的研究结果证实，阴道前壁远端三分之一区域的神经数量明显更多，性激素可能对这些显著差异有一定程度的影响。然而，雌激素在阴道神经支配中的作用仍存在争议。为了研究阴道中是否发生激素 - 神经相互作用，将60只大鼠随机分为六组：假手术组、卵巢切除组和4个治疗组。治疗2周后，用苏木精和伊红以及免疫组织化学方法检测PGP9.5制备阴道活检标本。完整阴道壁远端一半区域的小神经纤维密度明显高于近端一半区域（P = 0.001）。相反，接受手术绝经的去卵巢大鼠中，总的PGP 9.5免疫反应性纤维神经支配密度明显降低。持续给予雌激素2周导致神经纤维增生，在低剂量戊酸雌二醇组中数值达到正常水平。我们的研究结果表明，低剂量戊酸雌二醇全身激素治疗对于治疗绝经后女性因雌激素活性水平低引起的阴道神经支配不足是有效且安全的，并且可能有助于确定最佳雌二醇剂量以缓解阴道不适的研究。
