Research Centre for Biomedical Engineering, University of London, Northampton Square, London, EC1V 0HB, UK.
Analyst. 2017 May 15;142(10):1711-1719. doi: 10.1039/c7an00173h.
A fibre optic multi-sensor has been developed for biomedical sensing applications using a tip coating solution sensitive to both oxygen and carbon dioxide. An oxygen sensitive phosphorescence quenching complex based on platinum octaethylporphyrin (PtOEP) was combined with a carbon dioxide sensitive phosphorescence compound based on 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS). When excited by blue light (470 nm), the resultant coating had two fluorescent peaks at 515 nm (green) and 645 nm (red) which responded to partial pressure of CO and O respectively. The sensor was tested in vitro and shown to be able to measure CO and O simultaneously and in real time, with calibration constants of 0.0384 kPa and 0.309 kPa respectively. The O sensitive peak received some overlap from the 515 nm peak (0.38% of peak intensity) as well as some cross-sensitivity (maximum, 5.1 kPa pCO gave a measurement equivalent to 0.43 kPa of O, a ratio of 0.08 : 1). However, these effects can be subtracted from measurements and no significant cross-sensitivity or overlap was seen in CO measurements from O. This novel compound presents great potential for use in medical sensors and we expect it to be important to a wide range of future applications.
一种光纤多传感器已被开发用于生物医学传感应用，使用对氧气和二氧化碳都敏感的尖端涂层溶液。一种基于铂八乙基卟啉（PtOEP）的氧敏磷光猝灭配合物与基于 8-羟基芘-1,3,6-三磺酸三钠盐（HPTS）的二氧化碳敏磷光化合物结合。当用蓝光（470nm）激发时，所得涂层在 515nm（绿色）和 645nm（红色）处有两个荧光峰，分别对应于 CO 和 O 的分压。该传感器在体外进行了测试，能够实时同时测量 CO 和 O，校准常数分别为 0.0384kPa 和 0.309kPa。O 敏感峰受到来自 515nm 峰的一些重叠（峰强度的 0.38%）以及一些交叉敏感性（最大，5.1kPa pCO 给出的测量值相当于 0.43kPa 的 O，比值为 0.08:1）。然而，这些影响可以从测量中减去，并且在 O 中的 CO 测量中没有看到明显的交叉敏感性或重叠。这种新型化合物在医学传感器中具有很大的应用潜力，我们预计它将对未来的广泛应用非常重要。
