EIN2 介导的信号转导参与了拟南芥对晚疫病菌（Phytophthora infestans）的入侵前防御。
EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans.
机构信息
a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
b Graduate School of Agriculture , Shinshu University , Nagano , Japan.
出版信息
Plant Signal Behav. 2017 Apr 3;12(4):e1300733. doi: 10.1080/15592324.2017.1300733.
Abstract
Nicotiana benthamiana ABCG1 and ABCG2 are ABC transporters which are probably involved in the export of capsidiol, the major phytoalexin of Nicotiana species. While capsidiol export by these transporters plays an essential role in post-invasion defense against Phytophthora infestans, they also export unidentified antimicrobial compound(s) involved in pre-invasion defense. In this study, promoter activity of NbABCG2 (Pabcg2a) was analyzed using a GFP marker. Expression of GFP under the control of Pabcg2a was significantly increased by co-expression with the INF1 elicitor from P. infestans. Disruption of the ethylene-responsive GCC box in Pabcg2a compromised INF1-induced activation of Pabcg2a. Consistently, penetration by P. infestans was increased by gene-silencing of NbEIN2, the key ethylene-signaling component, suggesting the involvement of ethylene for pre-invasion defense of N. benthamiana.
摘要
烟草原生质体 ABCG1 和 ABCG2 是 ABC 转运蛋白，可能参与了贝壳杉烯醇的输出，贝壳杉烯醇是烟草原生质体的主要植物抗毒素。虽然这些转运蛋白对出口贝壳杉烯醇在入侵后抵抗致病疫霉方面起着至关重要的作用，但它们也出口参与入侵前防御的未鉴定的抗菌化合物。在这项研究中，使用 GFP 标记物分析了 NbABCG2（Pabcg2a）的启动子活性。在 P. infestans 的 INF1 激发子的共表达下，GFP 在 Pabcg2a 控制下的表达显著增加。在 Pabcg2a 中破坏乙烯反应性 GCC 框削弱了 INF1 诱导的 Pabcg2a 激活。一致地，通过基因沉默烟草原生质体中的关键乙烯信号成分 NbEIN2，致病疫霉的穿透增加，表明乙烯参与了烟草原生质体的入侵前防御。
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