Yeo Myung Gu, Kim Geun Hyung
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, Republic of Korea.
Biofabrication. 2017 Apr 12;9(2):025004. doi: 10.1088/1758-5090/aa6997.
In the cell-printing process, bioink has been considered as an extremely important component for successful fabrication of macroscale cell-laden structures. Bioink should be non-toxic, biocompatible, and printable. To date, alginate has been widely used as a whole or partial component of bioink because it is non-toxic to embedded cells and even it can provide good printability with rapid gelation under calcium ions. However, alginate bioinks do not possess cell-activating ability. To overcome the shortcomings of alginate-based bioinks, a new collagen bioink, which was mixed with human adipose stem cells (hASCs) and crosslinked with a polyphenol (tannic acid), was proposed. The feasibility of the bioink was demonstrated using several in vitro assessments for comparison of the macroscale porous cell-laden collagen/polyphenol structure containing the hASCs with the conventional alginate-based cell-laden structure. The levels of the metabolic activity, including the cell viability and cell proliferation, of the cell-laden collagen structure were significantly higher than those of the control (alginate-based cell-laden structure). The results show that the newly designed bioink and cell-laden structure are potentially new outstanding components for regeneration of various tissues.
在细胞打印过程中，生物墨水被视为成功制造宏观尺度载细胞结构的极其重要的组成部分。生物墨水应无毒、具有生物相容性且可打印。迄今为止，海藻酸盐已被广泛用作生物墨水的全部或部分成分，因为它对包埋的细胞无毒，甚至在钙离子存在下能通过快速凝胶化提供良好的可打印性。然而，海藻酸盐生物墨水不具备细胞激活能力。为克服基于海藻酸盐的生物墨水的缺点，一种新的胶原蛋白生物墨水被提出，它与人类脂肪干细胞（hASCs）混合并用一种多酚（单宁酸）交联。通过几种体外评估来证明这种生物墨水的可行性，以比较含有hASCs的宏观多孔载细胞胶原蛋白/多酚结构与传统的基于海藻酸盐的载细胞结构。载细胞胶原蛋白结构的代谢活性水平，包括细胞活力和细胞增殖，显著高于对照组（基于海藻酸盐的载细胞结构）。结果表明，新设计的生物墨水和载细胞结构可能是用于各种组织再生的新的杰出成分。
