Luo Gao-Quan, Liu Ling, Gao Qu-Wen, Wu Xiao-Na, Xiang Wei, Deng Wen-Ting
a Department of Neurology , Guangzhou General Hospital of Guangzhou Military Command , Guangdong , China.
b Department of The Geriatric Ward , Guangzhou General Hospital of Guangzhou Military Command , Guangdong , China.
Neurol Res. 2017 Aug;39(8):709-718. doi: 10.1080/01616412.2017.1310705. Epub 2017 Apr 12.
In recent years, a substantial amount of experimental studies have demonstrated that exogenous administration of corticosterone causes anxiety and depressive-like behaviour in rodents which involves hypothalamic-pituitary-adrenal axis dysregulation. Our present study aimed to explore the neuroprotective potential of mangiferin against corticosterone-induced anxiety and depressive-like behaviour.
Corticosterone (40 mg/kg; subcutaneously) was administered once daily in swiss albino mice for 21 days. Mice were treated simultaneously with mangiferin (40 mg/kg; p.o.), 30 min prior to the corticosterone injection.
Chronic administration of corticosterone caused anxiety and depressive-like behaviour in mice which was significantly alleviated by mangiferin treatment. Biochemical analysis revealed that mangiferin treatment significantly attenuated corticosterone-induced oxido-nitrosative stress and neuroinflammation in the hippocampus region. Furthermore, concomitant treatment with mangiferin significantly enhanced the hippocampal brain-derived neurotrophic factor (BDNF) level and decreased the serum corticosterone level in the corticosterone-treated animals. Western blotting analysis revealed that corticosterone administration significantly up-regulated the indoleamine 2,3-dioxygenase (IDO) protein expression level in the hippocampus which was significantly reduced by mangiferin treatment.
Taken together, our results suggest that mangiferin exerts anti-anxiety and antidepressant effect in corticosterone-treated rats, which is probably mediated through up-regulation of BDNF level along with inhibition of oxido-nitrosative stress, neuroinflammation and IDO up-regulation in the hippocampus region.
近年来，大量实验研究表明，外源性给予皮质酮会导致啮齿动物出现焦虑和抑郁样行为，这涉及下丘脑 - 垂体 - 肾上腺轴失调。我们目前的研究旨在探讨芒果苷对皮质酮诱导的焦虑和抑郁样行为的神经保护潜力。
将皮质酮（40毫克/千克；皮下注射）每日一次给予瑞士白化小鼠，持续21天。在注射皮质酮前30分钟，同时用芒果苷（40毫克/千克；口服）对小鼠进行治疗。
长期给予皮质酮会导致小鼠出现焦虑和抑郁样行为，而芒果苷治疗可显著减轻这种行为。生化分析表明，芒果苷治疗可显著减轻皮质酮诱导的海马区氧化 - 亚硝化应激和神经炎症。此外，在接受皮质酮治疗的动物中，同时给予芒果苷可显著提高海马脑源性神经营养因子（BDNF）水平并降低血清皮质酮水平。蛋白质印迹分析显示，给予皮质酮会显著上调海马中吲哚胺2,3 - 双加氧酶（IDO）蛋白表达水平，而芒果苷治疗可显著降低该水平。
综上所述，我们的结果表明，芒果苷在皮质酮处理的大鼠中发挥抗焦虑和抗抑郁作用，这可能是通过上调BDNF水平以及抑制海马区氧化 - 亚硝化应激、神经炎症和IDO上调来介导的。
