一种测定葡萄糖-1,6-二磷酸酶的方法。
A method for determination of glucose 1,6-bisphosphatase.
作者信息
Bassols A M, Carreras J, Cussó R
机构信息
Department de Ciències Fisiològiques Humanes i de la Nutrició, Facultat de Medicina, Universitat de Barcelona, Spain.
出版信息
J Biochem Biophys Methods. 1988 May;16(1):55-61. doi: 10.1016/0165-022x(88)90103-0.
PMID:2840460
Abstract
A sensitive and specific method to measure glucose 1,6-bisphosphatase activity, which allows the identification of the reaction products is described. [U-14 C]Glucose 1,6-P2, synthesized by the glucose 1-P kinase activity of phosphofructokinase, is used as substrate. The reaction products are separated and identified by chromatography on ion-exchange paper.
摘要
描述了一种灵敏且特异的测量葡萄糖1,6 - 二磷酸酶活性的方法，该方法能够鉴定反应产物。由磷酸果糖激酶的葡萄糖1 - 磷酸激酶活性合成的[U - ¹⁴C]葡萄糖1,6 - P₂用作底物。通过离子交换纸色谱法分离并鉴定反应产物。
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