Zack P M, Kociba G J
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Int J Cell Cloning. 1988 May;6(3):192-208. doi: 10.1002/stem.5530060305.
Pharmacologic modulators of cyclic 3',5'-adenosine monophosphate (cAMP) and calcium were added to cultures of bone marrow cells from normal cats and cats with retrovirus-induced erythroid aplasia (EA). Treatment with the following reagents increased the number of erythroid progenitors (CFU-e and BFU-e) in cells from normal cats: isoproterenol, dibutyryl cAMP, forskolin, RO-20-1724 and A23187. However, treatment of cells collected from viremic cats not only failed to enhance CFU-e and BFU-e but inhibited their growth. These studies suggest that EA is related to a non-reversible block of primitive erythroid progenitors or to direct inhibition of BFU-e and CFU-e growth.
将环3',5'-腺苷单磷酸（cAMP）和钙的药理学调节剂添加到正常猫和患有逆转录病毒诱导的红细胞生成障碍（EA）的猫的骨髓细胞培养物中。用以下试剂处理可增加正常猫细胞中红系祖细胞（CFU-e和BFU-e）的数量：异丙肾上腺素、二丁酰cAMP、福斯高林、RO-20-1724和A23187。然而，用从病毒血症猫收集的细胞进行处理不仅未能增强CFU-e和BFU-e，反而抑制了它们的生长。这些研究表明，EA与原始红系祖细胞的不可逆阻滞或对BFU-e和CFU-e生长的直接抑制有关。
