Li Hui, Wei Xuefeng, Yang Jiameng, Dong Dong, Huang Yongzhen, Lan Xianyong, Plath Martin, Lei Chuzhao, Qi Xinglei, Bai Yueyu, Chen Hong
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
Bureau of Animal Husbandry of Biyang County, Biyang, Henan 463700, China.
Oncotarget. 2017 May 9;8(19):32083-32100. doi: 10.18632/oncotarget.16644.
The formation of bovine skeletal muscle involves complex developmental and physiological processes that play a vital role in determining the quality of beef; however, the regulatory mechanisms underlying differences in meat quality are largely unknown. We conducted transcriptome analysis of bovine muscle tissues to compare gene expression profiles between embryonic and adult stages. Total RNAs from skeletal muscle of Qinchuan cattle at fetal and adult stages were used to construct libraries for Illumina next-generation sequencing using the Ribo-Zero RNA sequencing (RNA-Seq) method. We found a total of 19,695 genes to be expressed in fetal and adult stages, whereby 3,299 were expressed only in fetal, and 433 only in adult tissues. We characterized the role of a candidate gene (GosB), which was highly (but differentially) expressed in embryonic and adult skeletal muscle tissue. GosB increased the number of myoblasts in the S-phase of the cell cycle, and decreased the proportion of cells in the G0/G1 phase. GosB promoted the proliferation of myoblasts and protected them from apoptosis via regulating Bcl-2 expression and controlling the intracellular calcium concentration. Modulation of GosB expression in muscle tissue may emerge as a potential target in breeding strategies attempting to alter myoblast numbers in cattle.
牛骨骼肌的形成涉及复杂的发育和生理过程，这些过程在决定牛肉品质方面起着至关重要的作用；然而，肉质差异背后的调控机制在很大程度上尚不清楚。我们对牛肌肉组织进行了转录组分析，以比较胚胎期和成年期之间的基因表达谱。利用Ribo-Zero RNA测序（RNA-Seq）方法，将秦川牛胎儿期和成年期骨骼肌的总RNA用于构建文库，进行Illumina下一代测序。我们发现共有19695个基因在胎儿期和成年期表达，其中3299个仅在胎儿期表达，433个仅在成年组织中表达。我们对一个候选基因（GosB）的作用进行了表征，该基因在胚胎期和成年期骨骼肌组织中高表达（但存在差异）。GosB增加了细胞周期S期的成肌细胞数量，并降低了G0/G1期细胞的比例。GosB通过调节Bcl-2表达和控制细胞内钙浓度来促进成肌细胞的增殖并保护它们免于凋亡。调节肌肉组织中GosB的表达可能成为旨在改变牛成肌细胞数量的育种策略中的一个潜在靶点。
