F344大鼠的血管组织（钠，钾）ATP酶活性与衰老

Vascular tissue (Na,K)ATPase activity and aging in the F344 rat.

作者信息

Hennessy J F

机构信息

Bowman Gray School of Medicine, Wake Forest University, Department of Medicine/Endocrionology, Winston-Salem, NC 27103.

出版信息

Mech Ageing Dev. 1988 May;43(2):153-9. doi: 10.1016/0047-6374(88)90043-7.

DOI:10.1016/0047-6374(88)90043-7

PMID:2840535

Abstract

Vascular tissue (heart, thoracic aorta and tail artery) was removed from Fischer 344 rats, 12, 18 and 27 months of age. The intact tissue was then used to determine total, ouabain-sensitive and ouabain-insensitive 86Rubidium (86Rb) uptakes, to provide a reflection of (Na,K)ATPase activity. These studies indicate no change in total, ouabain-sensitive nor ouabain-insensitive 86Rb uptakes into cardiac tissue isolated from these rats. However, tail artery total and ouabain-sensitive 86Rb uptake decreased with age (a 61% decrease in ouabain-sensitive 86Rb uptake in 12 vs. 27-month-old rats) without significant changes in the ouabain-insensitive 86Rb uptake. This pattern was repeated in aortic tissue with a 56% decrease in ouabain-sensitive 86Rb uptake in 12 vs. 27-month-old rats. The results of these studies support an age-related decline in (Na,K)ATPase activity in aortic and tail artery tissue without a significant change in cardiac (Na,K)ATPase activity between 12, 18 and 27-month-old Fischer 344 rats.

摘要

从12、18和27月龄的Fischer 344大鼠身上取出血管组织（心脏、胸主动脉和尾动脉）。然后使用完整组织来测定总的、哇巴因敏感的和哇巴因不敏感的86铷（86Rb）摄取量，以反映（钠，钾）ATP酶活性。这些研究表明，从这些大鼠分离出的心脏组织中，总的、哇巴因敏感的和哇巴因不敏感的86Rb摄取量均无变化。然而，尾动脉总的和哇巴因敏感的86Rb摄取量随年龄下降（12月龄与27月龄大鼠相比，哇巴因敏感的86Rb摄取量下降61%），而哇巴因不敏感的86Rb摄取量无显著变化。在主动脉组织中也出现了这种模式，12月龄与27月龄大鼠相比，哇巴因敏感的86Rb摄取量下降56%。这些研究结果支持了在12、18和27月龄的Fischer 344大鼠中，主动脉和尾动脉组织中（钠，钾）ATP酶活性随年龄下降，而心脏（钠，钾）ATP酶活性无显著变化。