Knowles D M, Pelicci P G, Dalla-Favera R
Department of Pathology, New York University School of Medicine, NY 10016.
Mol Cell Probes. 1987 Mar;1(1):15-31. doi: 10.1016/0890-8508(87)90004-1.
The application of DNA probes to demonstrate clonal rearrangements of the immunoglobulin heavy and light chain and T cell receptor beta chain gene loci by Southern blot hybridization analysis has led to significant advances in our ability to diagnose, classify and investigate the lymphoproliferative disorders. This approach has allowed us to conclusively determine the B or T cell lineage derivation and the clonal nature of the vast majority of lymphoid neoplasms, resulting in a new level of understanding of the biology of lymphoid neoplasia. Further application of these DNA probes to other poorly defined and controversial lymphoproliferative disorders should clarify their nature as well. Eventually, antigen receptor gene rearrangement analysis may become routinely employed in diagnosing lymphoid malignancies, monitoring the effects of chemotherapy, detecting early subclinical relapses and identifying disease progression, especially with respect to detecting the emergence of new clonal populations.
通过Southern印迹杂交分析应用DNA探针来证明免疫球蛋白重链和轻链以及T细胞受体β链基因座的克隆重排，已使我们在诊断、分类和研究淋巴增殖性疾病的能力方面取得了重大进展。这种方法使我们能够最终确定绝大多数淋巴样肿瘤的B或T细胞谱系来源以及克隆性质，从而对淋巴样肿瘤的生物学有了新的认识。将这些DNA探针进一步应用于其他定义不明确且有争议的淋巴增殖性疾病，也应能阐明它们的性质。最终，抗原受体基因重排分析可能会常规用于诊断淋巴恶性肿瘤、监测化疗效果、检测早期亚临床复发以及识别疾病进展，特别是在检测新克隆群体的出现方面。
