Majumdar Jayjeet, Chakraborty Pratip, Mitra Analava, Sarkar Nirmal Kumar, Sarkar Supriti
Department of Zoology, City College, 102/1 Raja Rammohan Sarani, Kolkata, Affiliated to University of Calcutta, West Bengal, India.
School of Medical Science & Technology, Indian Institute of Technology, Kharagpur, Kharagpur, West Bengal, India.
Exp Clin Endocrinol Diabetes. 2017 Jul;125(7):441-448. doi: 10.1055/s-0043-103458. Epub 2017 Apr 13.
Fenugreek (, a medicinal herb with potent antihyperglycaemic and hypoglycaemic effects, is used to treat diabetes. This study is aimed to explore the interaction of fenugreek seed extract (FSE) and HPT (hypothalamic-pituitary-thyroid) axis in context of leptin secretion which have important role in normal and type-1 diabetic subjects. FSE (confirmed to contain trigonelline, diosgenin, 4 hydroxyisoleucine) was gavaged (0.25 gm/kg body weight/day) to normal and alloxan-induced type-1 diabetic rats for 4 weeks. Expression of hypothalamic prepro-TRH (Thyrotropin releasing hormone) mRNA, serum levels of TRH, TSH (Thyroid stimulating hormone), fT, fT, insulin, leptin, glucose; thyroperoxidase activity and growth of thyroid gland, food intake, adiposity index were also studied FSE significantly down regulated prepro-TRH mRNA expression; decreased serum TRH, TSH, fT, fT levels, and regressed thyroid gland in FSE-fed normal and diabetic rats than those observed in normal diet-fed control and diabetic rats. FSE decreased (p<0.005-0.001) adiposity index and leptin secretion, increased food intake and body weight in all FSE-fed rats. FSE improved insulin secretion, decreased glucose level but impaired HPT axis in diabetic rats, indicating insulin-independent central hypothyroidism. Results suggested that the dominant signal to hypothalamus suppressing HPT axis is the fall in leptin level which i resulted from decreased adiposity index following FSE feeding. Fenugreek simultaneously having hypoglycaemic and hypothyroidal actions raises questions whether it can be safely used to treat diabetes and/or hyperthyroidism as was suggested by many workers.
胡芦巴是一种具有强大降血糖和低血糖作用的药草，用于治疗糖尿病。本研究旨在探讨胡芦巴种子提取物（FSE）与下丘脑 - 垂体 - 甲状腺（HPT）轴在瘦素分泌方面的相互作用，瘦素分泌在正常和1型糖尿病受试者中具有重要作用。将FSE（经证实含有胡芦巴碱、薯蓣皂苷元、4 - 羟基异亮氨酸）以0.25克/千克体重/天的剂量灌胃给正常和四氧嘧啶诱导的1型糖尿病大鼠，持续4周。还研究了下丘脑前促甲状腺激素释放激素（TRH）mRNA的表达、血清中TRH、促甲状腺激素（TSH）、游离甲状腺素（fT）、游离三碘甲状腺原氨酸（fT）、胰岛素、瘦素、葡萄糖的水平；甲状腺过氧化物酶活性以及甲状腺的生长、食物摄入量、肥胖指数。与正常饮食喂养的对照大鼠和糖尿病大鼠相比，FSE显著下调了前促甲状腺激素释放激素mRNA的表达；降低了FSE喂养的正常和糖尿病大鼠的血清TRH、TSH、fT、fT水平，并使甲状腺腺体萎缩。FSE降低了（p<0.005 - 0.001）所有FSE喂养大鼠的肥胖指数和瘦素分泌，增加了食物摄入量和体重。FSE改善了糖尿病大鼠的胰岛素分泌，降低了血糖水平，但损害了HPT轴，表明存在胰岛素非依赖性中枢性甲状腺功能减退。结果表明，抑制HPT轴的下丘脑主要信号是瘦素水平的下降，这是由于FSE喂养后肥胖指数降低所致。许多研究人员认为胡芦巴同时具有降血糖和甲状腺功能减退作用，这引发了关于它是否能安全用于治疗糖尿病和/或甲状腺功能亢进症的疑问。
