Huang Zuhao, Ruan Rujue
a School of Life Sciences, Jinggangshan University , Ji'an , Jiangxi Province , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 May;29(4):529-534. doi: 10.1080/24701394.2017.1315569. Epub 2017 Apr 13.
DNA barcoding has become a promising tool for species identification and phylogeny in a wide range of animal taxa using mitochondrial cytochrome c oxidase subunit I (COI). The Corvidae (Aves: Passeriformes) is a species rich and morphologically diverse family. In the present study, we analyzed the COI barcodes of 39 species from 12 genera of Corvidae. COI gene was also used to examine phylogenetic relationships of Corvidae. Every species possessed a barcode distinct from that of other species. Kimura two-parameter distances were calculated between species barcodes. The average genetic distance between the species was 22 times higher compared to the average genetic distance within species. Maximum likelihood method was used to construct a phylogenetic tree. All the species could be discriminated by their distinct clades in the phylogenetic tree. COI gene data provided good evidence for the monophyly of the Corvidae. Members of Cyanopica and Pyrrhocorax were the first to split from the Corvidae lineage. Analysis of COI genes supported the others genera fell into two clades. DNA barcoding is an effective molecular tool for Corvidae species identification and phylogenetic inference.
DNA条形码已成为一种很有前景的工具，可利用线粒体细胞色素c氧化酶亚基I（COI）对广泛的动物类群进行物种鉴定和系统发育研究。鸦科（鸟类：雀形目）是一个物种丰富且形态多样的科。在本研究中，我们分析了鸦科12个属39个物种的COI条形码。COI基因也被用于研究鸦科的系统发育关系。每个物种都拥有一个与其他物种不同的条形码。计算了物种条形码之间的Kimura双参数距离。物种之间的平均遗传距离比物种内的平均遗传距离高22倍。采用最大似然法构建系统发育树。在系统发育树中，所有物种都可以通过其独特的分支进行区分。COI基因数据为鸦科的单系性提供了有力证据。蓝鹊属和红嘴山鸦属的成员最先从鸦科谱系中分化出来。对COI基因的分析支持其他属分为两个分支。DNA条形码是鸦科物种鉴定和系统发育推断的有效分子工具。
