Zhang Haipeng, Xie Yunxia, Liu Cuihua, Chen Shilin, Hu Shuangshuang, Xie Zongzhou, Deng Xiuxin, Xu Juan
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Collene of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Food Chem. 2017 Sep 1;230:316-326. doi: 10.1016/j.foodchem.2017.03.040. Epub 2017 Mar 10.
The volatile profiles of fruit peels and juice sacs from 108 citrus accessions representing seven species were analyzed. Using GC-MS 162 and 107 compounds were determined in the peels and juice sacs, respectively. In the peels, monoterpene alcohols were accumulated in loose-skin mandarins; clementine tangerines and papedas were rich in sesquiterpene alcohols, sesquiterpenes, monoterpene alcohols and monoterpene aldehydes. β-pinene and sabinene were specifically accumulated in 4 of 5 lemon germplasms. Furthermore, concentrations of 34 distinctive compounds were selected to best represent the volatile profiles of seven species for HCA analysis, and the clustering results were in agreement with classic citrus taxonomy. Comparison of profiles from different growing seasons and production areas indicated that environmental factors play important roles in volatile metabolism. In addition, a few citrus germplasms that accumulated certain compounds were determined as promising breeding materials. Notably, volatile biosynthesis via MVA pathway in C. ichangensis 'Huaihua' was enhanced.
对代表七个物种的108份柑橘种质的果皮和汁囊的挥发性成分进行了分析。使用气相色谱 - 质谱联用仪分别在果皮和汁囊中鉴定出162种和107种化合物。在果皮中，单萜醇在薄皮柑橘中积累；克莱门氏小柑橘和巴皮斯柑橘富含倍半萜醇、倍半萜、单萜醇和单萜醛。β-蒎烯和桧烯在5份柠檬种质中的4份中特异性积累。此外，选择了34种独特化合物的浓度以最好地代表七个物种的挥发性成分用于聚类分析，聚类结果与经典柑橘分类法一致。不同生长季节和产地的成分比较表明，环境因素在挥发性代谢中起重要作用。此外，一些积累特定化合物的柑橘种质被确定为有前途的育种材料。值得注意的是，宜昌橙‘怀化’中通过甲羟戊酸途径的挥发性生物合成增强。
