Wu Chueh-Hung, Sun Ming-Kuan, Shieh Jay, Chen Chuin-Shan, Huang Chang-Wei, Dai Chi-An, Chang Shu-Wei, Chen Wen-Shiang, Young Tai-Horng
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, Taipei, Taiwan.
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, Taiwan.
Ultrasonics. 2018 Feb;83:157-163. doi: 10.1016/j.ultras.2017.03.019. Epub 2017 Apr 2.
Episodic release of bioactive compounds plays an important role in biological systems. "On-demand" release systems which based on polymeric materials and activated by external stimuli may provide the necessary functionality. Here we describe an ultrasound-responsive hydrogel based on N-isopropylacrylamide (NIPAM) and N,N'-methylenebisacrylamide (MBAm), which is suitable for triggered release of two large molecules: bovine serum albumin (BSA, 66kDa) and dextran (3-5kDa). It is shown that the release amount of these two large molecules increased with increasing hydrogel temperature, and the application of ultrasound further increased the release. By simply adjusting the contents of NIPAM and MBAm, the difference of BSA release between the presence and absence of ultrasound could be adjusted from 2.7 to 84 folds. There was also a positive correlation between the ultrasound intensity and release amount. These properties made the NIPAM-based hydrogel a tunable platform for focal drug delivery.
生物活性化合物的间歇性释放在生物系统中起着重要作用。基于聚合物材料并由外部刺激激活的“按需”释放系统可能提供必要的功能。在此，我们描述了一种基于N-异丙基丙烯酰胺（NIPAM）和N，N'-亚甲基双丙烯酰胺（MBAm）的超声响应水凝胶，它适用于两种大分子的触发释放：牛血清白蛋白（BSA，66kDa）和葡聚糖（3-5kDa）。结果表明，这两种大分子的释放量随水凝胶温度的升高而增加，超声的应用进一步增加了释放量。通过简单地调整NIPAM和MBAm的含量，有无超声时BSA释放的差异可从2.7倍调整到84倍。超声强度与释放量之间也存在正相关。这些特性使基于NIPAM的水凝胶成为用于局部药物递送的可调平台。
