Oddsdottir M, Goldenring J R, Adrian T E, Zdon M J, Zucker K A, Modlin I M
Department of Surgery, Yale University School of Medicine, West Haven, Connecticut.
Biochem Biophys Res Commun. 1988 Jul 29;154(2):489-96. doi: 10.1016/0006-291x(88)90166-0.
Histamine stimulated acid secretion is mediated by an increase in intracellular cAMP. Cytosolic protein phosphorylation stimulated by histamine was investigated in isolated rabbit parietal cells. Histamine stimulated the phosphorylation of a 30 kDa phosphoprotein with an isoelectric point of 5.6. Cimetidine completely inhibited histamine-stimulated pp30 phosphorylation. However, omeprazole had no effect on the phosphorylation of pp30. Forskolin and 8-bromo-cAMP also stimulated the phosphorylation of pp30. The results suggest that pp30 is a histamine-stimulated, cAMP-dependently phosphorylated protein substrate in parietal cell cytosol.
