培养细胞中缩醛磷脂合成的分析
Analysis of Plasmalogen Synthesis in Cultured Cells.
作者信息
Honsho Masanori, Fujiki Yukio
机构信息
Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
出版信息
Methods Mol Biol. 2017;1595:55-61. doi: 10.1007/978-1-4939-6937-1_6.
PMID:28409451
Abstract
Plasmalogen synthesis can be analyzed by metabolic labeling, followed by the separation of ethanolamine plasmalogens from glycerophospholipids on one-dimensional thin-layer chromatography. The vinyl-ether bond of plasmalogens is acid-labile, which allows separating plasmalogens as 2-acyl-glycerophospholipids from diacyl-glycerophospholipids.
摘要
可以通过代谢标记分析缩醛磷脂的合成，随后在一维薄层色谱上从甘油磷脂中分离出乙醇胺缩醛磷脂。缩醛磷脂的乙烯基醚键对酸不稳定，这使得缩醛磷脂能够作为2-酰基甘油磷脂与二酰基甘油磷脂分离。
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