Punia Harish, Gathwala Geeta, Dhaulakhandi Dhara B, Aamir Mohammed
1 Senior resident, Department of Paediatrics, All India Institute of Medical Sciences, New Delhi, India.
2 Senior professor and head, Department of Paediatrics, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.
Trop Doct. 2017 Oct;47(4):336-339. doi: 10.1177/0049475517701875. Epub 2017 Apr 14.
The gold standard for detecting bacterial sepsis is blood culture. However, the sensitivity of blood culture is low and the results take 48-72 h. Molecular assays for the detection of bacterial DNA permit early detection of a bacterial cause as the turnaround time is 6-8 h. We undertook an evaluation of the performance of universal bacterial primer (16S rRNA) polymerase chain reaction (PCR) in the diagnosis of neonatal sepsis at a tertiary care medical college teaching hospital. 16S rRNA PCR was positive in all cases of blood culture proven sepsis. PCR revealed 95.6% sensitivity, 100% specificity, 100% positive predictive value and 91.2% negative predictive value and so appears to be a useful tool for the early diagnosis of bacterial neonatal sepsis.
检测细菌性败血症的金标准是血培养。然而，血培养的敏感性较低，结果需要48 - 72小时才能得出。检测细菌DNA的分子检测方法能够实现早期检测细菌病因，因为其周转时间为6 - 8小时。我们在一家三级医疗学院教学医院对通用细菌引物（16S rRNA）聚合酶链反应（PCR）在新生儿败血症诊断中的性能进行了评估。在所有血培养证实为败血症的病例中，16S rRNA PCR均呈阳性。PCR显示出95.6%的敏感性、100%的特异性、100%的阳性预测值和91.2%的阴性预测值，因此似乎是早期诊断新生儿细菌性败血症的一种有用工具。
