Korczyński Jaroslaw, Kubiak Katarzyna, Węgłowska Edyta
Scientific Research Advisory Centre Kawa.ska Sp. z o.o. 5 Techniczna St., 05-500 Piaseczno, Poland.
Molecular Biotechnology Laboratory, Bionanopark Sp. z o.o., 114/116 Dubois St., 93-465 Lodz, Poland.
Postepy Biochem. 2017;63(1):44-52.
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is an imaging method for living cells visualization as well as for food or cosmetics material analysis without the need for staining. The near infrared laser source generates the CARS signal - the characteristic intrinsic vibrational contrast of the molecules in a sample which is no longer caused by staining, but by the molecules themselves. It provides the benefit of a non-toxic, non-destructive and almost noninvasive method for sample imaging. CARS can easily be combined with fluorescence confocal microscopy so it is an excellent complementary imaging method. In this article we showed some of the applications for this technology: imaging of lipid droplets inside human HaCaT cells and analysis of the composition of cosmetic products. Moreover we believe, that soon new fields of application become accessible for this rapidly developing branch of microscopy.
相干反斯托克斯拉曼散射（CARS）显微镜是一种用于活细胞可视化以及食品或化妆品材料分析的成像方法，无需染色。近红外激光源产生CARS信号——样品中分子的特征性固有振动对比度，这不再是由染色引起的，而是由分子本身引起的。它提供了一种无毒、非破坏性且几乎无创的样品成像方法。CARS可以很容易地与荧光共聚焦显微镜结合，因此它是一种出色的互补成像方法。在本文中，我们展示了这项技术的一些应用：对人HaCaT细胞内脂滴的成像以及化妆品成分分析。此外，我们相信，对于这一快速发展的显微镜分支领域，很快就会有新的应用领域出现。
