Hutson P A, Holgate S T, Church M K
Immunopharmacology Group, Southampton General Hospital.
Br J Pharmacol. 1988 May;94(1):6-8. doi: 10.1111/j.1476-5381.1988.tb11493.x.
Bronchial challenge of ovalbumin-sensitized conscious guinea-pigs induced a triphasic reduction in specific airways conductance (sGaw) with maximal reductions being observed at 2, 17 and 72 h accompanied by infiltration of the airways with neutrophils at 17 h and eosinophils at 17 and 72 h. Nedocromil sodium (10 mg ml-1) inhaled before challenge blocked the 2 and 17 h sGaw response and the neutrophil influx. When inhaled 6 h after challenge, nedocromil inhibited the 17 h sGaw response but not neutrophil influx, suggesting that these are unrelated. The sGaw response and eosinophil accumulation at 72 h was also inhibited by nedocromil given at this time. Our findings correlate with the clinical effects of nedocromil, suggesting the guinea-pig model may be useful for investigating the mechanism of action of anti-asthmatic drugs.
对卵清蛋白致敏的清醒豚鼠进行支气管激发试验，可导致特异性气道传导率（sGaw）呈三相降低，在2小时、17小时和72小时观察到最大降幅，同时在17小时气道有中性粒细胞浸润，在17小时和72小时有嗜酸性粒细胞浸润。激发试验前吸入奈多罗米钠（10 mg/ml）可阻断2小时和17小时的sGaw反应以及中性粒细胞流入。激发试验后6小时吸入时，奈多罗米抑制了17小时的sGaw反应，但未抑制中性粒细胞流入，提示二者无关。此时给予奈多罗米也可抑制72小时的sGaw反应和嗜酸性粒细胞聚集。我们的研究结果与奈多罗米的临床效果相关，提示豚鼠模型可能有助于研究抗哮喘药物的作用机制。
