Maksimova I V, Dal' E S
Mikrobiologiia. 1975 Nov-Dec;44(6):1057-63.
The maximum production of glycolic acid by the cells of Chlorella pyrenoidosa, strain S-39, was found when the cells, grown at the content of carbon dioxide of 2% in the air and illumination of 2000 lx, were transferred to 10(-3) M phosphate buffer (pH 7.0) with barbotage by pure oxygen and illumination of 30,000 lx. The rate of production of glycolic acid during the first five minutes is the same in the light and darkness; later no glycolate is formed in the darkness while in the light glycolate is formed at a lower rate. Bicarbonate inhibits production of glycolate. Negative interaction has been established between oxygen and bicarbonate during their effect on the production of glycolic acid. These data confirm the production of glycolic acid in the cells of Chlorella pyrenoidosa from ribulose diphosphate which is oxidized by molecular oxygen with the participation of ribulose diphosphate carboxylase acting as an oxygenase.
当在空气中二氧化碳含量为2%且光照为2000勒克斯条件下生长的小球藻（S-39品系）细胞，转移至含10⁻³M磷酸盐缓冲液（pH 7.0）、经纯氧鼓泡且光照为30000勒克斯的环境中时，发现其乙醇酸产量达到最大值。在前五分钟内，乙醇酸的生成速率在光照和黑暗条件下相同；之后在黑暗中不再形成乙醇酸，而在光照下乙醇酸以较低速率形成。碳酸氢盐会抑制乙醇酸的生成。已确定氧气和碳酸氢盐在影响乙醇酸生成过程中存在负相互作用。这些数据证实了小球藻细胞中乙醇酸是由二磷酸核酮糖生成的，二磷酸核酮糖在作为加氧酶的二磷酸核酮糖羧化酶参与下被分子氧氧化。
