Faull K F, Bowersox S S, Zeller-DeAmicis L, Maddaluno J F, Ciaranello R D, Dement W C
Pasarow Analytical Neurochemical Facility, Stanford University School of Medicine, CA 94305.
Brain Res. 1988 May 31;450(1-2):225-30. doi: 10.1016/0006-8993(88)91561-2.
Brains from breed and age-matched canines stored at -80 degrees C for between 3 and 44 months showed a time-dependent decline in the concentration of norepinephrine in the amygdala and dopamine and norepinephrine in the caudate. No changes were seen in the density or ligand affinities of prazosin or spiperone binding sites in the same areas nor were there changes in quinuclidinyl benzylate binding sites in the frontal cortex. The changes in dopamine concentrations in the caudate were not accompanied by changes in the concentrations of dopamine metabolites. The chromatograms from which the dopamine and norepinephrine concentrations were estimated contained several unidentified, amperometrically detectable, extraneous peaks which increased in size in the older tissue samples. These results suggest that the decline in dopamine and norepinephrine concentrations was not the result of enzymatic breakdown, but probably the result of chemical decomposition. These findings have significance for the measurement of dopamine and norepinephrine concentrations in autopsied brains kept frozen in storage.
在-80摄氏度下储存3至44个月的品种和年龄匹配的犬类大脑显示，杏仁核中去甲肾上腺素以及尾状核中多巴胺和去甲肾上腺素的浓度呈时间依赖性下降。在相同区域，哌唑嗪或螺哌隆结合位点的密度或配体亲和力没有变化，额叶皮质中苄基奎宁环酯结合位点也没有变化。尾状核中多巴胺浓度的变化并未伴随多巴胺代谢物浓度的变化。用于估算多巴胺和去甲肾上腺素浓度的色谱图包含几个未识别的、可通过安培法检测到的外来峰，这些峰在较老的组织样本中尺寸增大。这些结果表明，多巴胺和去甲肾上腺素浓度的下降不是酶促分解的结果，而可能是化学分解的结果。这些发现对于测量冷冻保存的尸检大脑中多巴胺和去甲肾上腺素的浓度具有重要意义。
