有限蛋白酶解后通过肽图谱分析比较两种不同的苯二氮䓬结合蛋白。

Comparison of two different benzodiazepine binding proteins by peptide mapping after limited proteolysis.

作者信息

Sieghart W

机构信息

Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik Wien, Vienna, Austria.

出版信息

Brain Res. 1988 May 31;450(1-2):387-91. doi: 10.1016/0006-8993(88)91581-8.

DOI:10.1016/0006-8993(88)91581-8

PMID:2841007

Abstract

Proteins P51 and P55 were photolabeled by [3H]flunitrazepam, [3H]clonazepam or 3H-Ro 15-4513 and then compared by peptide mapping after limited digestion with various proteases. Results indicated that [3H]flunitrazepam or [3H]clonazepam irreversibly bind to the same and [3H]Ro 15-4513 to a different part of these proteins. The different radiolabeled peptide patterns obtained from P51 or P55, irrespective of the photolabel used, suggest a difference in the molecular structure of these proteins.

摘要

蛋白质P51和P55用[³H]氟硝西泮、[³H]氯硝西泮或³H-Ro 15-4513进行光标记，然后在用各种蛋白酶进行有限消化后通过肽图谱进行比较。结果表明，[³H]氟硝西泮或[³H]氯硝西泮不可逆地结合到这些蛋白质的相同部位，而[³H]Ro 15-4513结合到不同部位。无论使用何种光标记，从P51或P55获得的不同放射性标记肽图谱表明这些蛋白质的分子结构存在差异。