Zhang Shaokun, Lv Zhenshan, Hu Yang, Liu Lidi, Gong Weiquan, Li Qiao, Wu Hong
Department of Spine Surgery, The 1st Hospital of Jilin University, Changchun 130021, China.
Nursing and Health Sciences, Faculty of Medicine, Monash University, Melbourne, Australia.
Stem Cell Res. 2017 Mar;19:49-51. doi: 10.1016/j.scr.2016.12.023. Epub 2016 Dec 21.
Peripheral blood was collected from a clinically diagnosed 64-year old male multiple schwannoma patient. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma.
从一名临床诊断为多发性神经鞘瘤的64岁男性患者采集外周血。使用仙台病毒重编程系统，用山中KMOS重编程因子对外周血单个核细胞（PBMC）进行重编程。通过对多能性标志物的免疫荧光染色验证，无转基因的诱导多能干细胞系表现出多能性，并且该诱导多能干细胞系能够在体内分化为三个胚层。该诱导多能干细胞系还显示出正常的核型。这种体外细胞模型将有助于多发性神经鞘瘤的进一步病理学研究。
