Marsoner Fabio, Marcatili Matteo, Karnavas Thodoris, Bottai Daniele, D'Agostino Armando, Scarone Silvio, Conti Luciano
Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy.
Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy; Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy.
Stem Cell Res. 2016 Nov;17(3):661-664. doi: 10.1016/j.scr.2016.11.005. Epub 2016 Nov 10.
Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.
外周血单个核细胞（PBMCs）取自一名对氯氮平耐药（也称为“超级难治性”）精神分裂症患者。使用基于仙台病毒的非整合重编程系统建立了诱导多能干细胞（iPSCs）。对一个无足迹的人诱导多能干细胞系进行了表征，结果显示其表达主要的内源性多能性标志物并保持正常核型。细胞通过产生属于三个胚层的分化细胞后代来显示多能性能力。这个人诱导多能干细胞系是一种有价值的工具，可在体外获得成熟的、与病理相关的神经元群体，适合于研究精神分裂症的分子背景以及患者对治疗的反应。
