用SV40病毒进行转化可防止视黄酸对大鼠肝细胞质膜NADH二价铁转铁蛋白还原酶的抑制作用。

Transformation with SV40 virus prevents retinoic acid inhibition of plasma membrane NADH diferric transferrin reductase in rat liver cells.

作者信息

Sun I L, Toole-Simms W, Crane F L, Morré D J, Löw H, Chou J Y

机构信息

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

出版信息

J Bioenerg Biomembr. 1988 Jun;20(3):383-91. doi: 10.1007/BF00769639.

DOI:10.1007/BF00769639

PMID:2841310

Abstract

Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown at the permissive 33 degrees C temperature), retinoic acid does not inhibit the diferric transferrin reduction. Inhibition of activity of nontransformed cells is specific for retinoic acid with only slight inhibition by retinol and retinyl acetate at higher concentrations. Isolated rat liver plasma membrane NADH diferric transferrin reductase is also inhibited by retinoic acid. The effect of transformation with SV40 virus to decrease susceptibility to retinoic acid inhibition stands in contrast to much greater adriamycin inhibition of diferric transferrin reduction in the transformed cells than in nontransformed cells.

摘要

当在40摄氏度培养处于未转化状态的、感染了温度敏感型SV40病毒的胎鼠肝细胞时，视黄酸通过跨质膜电子传递系统抑制三价铁转铁蛋白的还原。当细胞处于转化状态（在允许的33摄氏度温度下生长）时，视黄酸不抑制三价铁转铁蛋白的还原。视黄酸对未转化细胞活性的抑制具有特异性，只有在较高浓度下视黄醇和醋酸视黄酯才有轻微抑制作用。分离的大鼠肝质膜NADH三价铁转铁蛋白还原酶也受到视黄酸的抑制。SV40病毒转化导致细胞对视黄酸抑制的敏感性降低，这与阿霉素对转化细胞中三价铁转铁蛋白还原的抑制作用比未转化细胞中强得多形成对比。