Löw H, Sun I L, Navas P, Grebing C, Crane F L, Morre D J
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1117-23. doi: 10.1016/s0006-291x(86)80293-5.
Intact cells are known to reduce external, impermeable electron acceptors. We now show that cells can reduce the iron in diferric transferrin at the cell surface and that this reduction reaction depends on the transferrin receptor as well as the transmembrane electron transport system. Reduction of external diferric transferrin is accompanied by oxidation of internal NADH which indicates that the transmembrane enzyme is an NADH diferric transferrin reductase. Highly purified liver plasma membranes have NADH diferric transferrin reductase activity which shows properties similar to the diferric transferrin reductases activity of intact cells. Cell growth stimulation by diferric transferrin and other impermeable oxidants which can react with the diferric transferrin reductase can be based on electron transport through he plasma membrane.
已知完整细胞可还原外部不可渗透的电子受体。我们现在表明，细胞能够在细胞表面还原二价铁转铁蛋白中的铁，且这种还原反应依赖于转铁蛋白受体以及跨膜电子传输系统。外部二价铁转铁蛋白的还原伴随着内部NADH的氧化，这表明跨膜酶是一种NADH二价铁转铁蛋白还原酶。高度纯化的肝细胞膜具有NADH二价铁转铁蛋白还原酶活性，其表现出与完整细胞的二价铁转铁蛋白还原酶活性相似的特性。二价铁转铁蛋白和其他可与二价铁转铁蛋白还原酶反应的不可渗透氧化剂对细胞生长的刺激作用可能基于通过质膜的电子传输。
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