Shariff Khairul Anuar, Tsuru Kanji, Ishikawa Kunio
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, 14300, Nibong Tebal, Penang, Malaysia.
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:1411-1419. doi: 10.1016/j.msec.2017.03.004. Epub 2017 Mar 3.
β-Tricalcium phosphate (β-TCP) has attracted much attention as an artificial bone substitute owing to its biocompatibility and osteoconductivity. In this study, osteoconductivity of β-TCP bone substitute was enhanced without using growth factors or cells. Dicalcium phosphate dihydrate (DCPD), which is known to possess the highest solubility among calcium phosphates, was coated on β-TCP granules by exposing their surface with acidic calcium phosphate solution. The amount of coated DCPD was regulated by changing the reaction time between β-TCP granules and acidic calcium phosphate solution. Histomorphometry analysis obtained from histological results revealed that the approximately 10mol% DCPD-coated β-TCP granules showed the largest new bone formation compared to DCPD-free β-TCP granules, approximately 2.5mol% DCPD-coated β-TCP granules, or approximately 27mol% DCPD-coated β-TCP granules after 2 and 4weeks of implantation. Based on this finding, we demonstrate that the osteoconductivity of β-TCP granules could be improved by coating their surface with an appropriate amount of DCPD.
β-磷酸三钙（β-TCP）因其生物相容性和骨传导性作为一种人工骨替代物备受关注。在本研究中，未使用生长因子或细胞的情况下增强了β-TCP骨替代物的骨传导性。已知磷酸二钙二水合物（DCPD）在磷酸钙中具有最高的溶解度，通过用酸性磷酸钙溶液暴露β-TCP颗粒表面，将其涂覆在β-TCP颗粒上。通过改变β-TCP颗粒与酸性磷酸钙溶液之间的反应时间来调节涂覆的DCPD量。从组织学结果获得的组织形态计量学分析表明，与未涂覆DCPD的β-TCP颗粒、约2.5mol% DCPD涂覆的β-TCP颗粒或约27mol% DCPD涂覆的β-TCP颗粒相比，植入2周和4周后，约10mol% DCPD涂覆的β-TCP颗粒显示出最大的新骨形成。基于这一发现，我们证明通过在β-TCP颗粒表面涂覆适量的DCPD可以提高其骨传导性。
