Yang Bingxian, Guan Qijie, Tian Jingkui, Komatsu Setsuko
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China.
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
Data Brief. 2017 Mar 25;12:138-142. doi: 10.1016/j.dib.2017.03.021. eCollection 2017 Jun.
High level of UV-B irradiation followed by dark treatment accumulates secondary metabolites in DC. To investigate the response mechanism under high level of UV-B irradiation followed by dark treatment, transcriptomic and proteomic analyses were performed in leaves of DC. The experimental design for the transcriptomic and proteomic analyses in leaves of under stresses was organized into a picture. For transcriptomics, mRNA-sequencing technology was used. Genes identified in leaves of at starting point, high level of UV-B irradiation, and high level of UV-B irradiation followed by dark treatment were listed; genes with different expression levels at starting point, high level of UV-B irradiation, and high level of UV-B irradiation followed by dark treatment were also presented in this DiB article. For proteomics, a gel-free/label-free proteomic technique was used. Proteins with different abundances in leaves at starting point, high level of UV-B irradiation, and high level of UV-B irradiation followed by dark treatment were presented in this DiB article. In order to monitor the expression levels of genes under the stress, quantitative reverse transcription polymerase chain reaction was performed. The primer sequences of genes selected for quantitative reverse transcription polymerase chain reaction was presented in this DiB article.
高强度UV-B照射后进行黑暗处理会使二色补血草积累次生代谢产物。为了研究高强度UV-B照射后进行黑暗处理的响应机制,对二色补血草叶片进行了转录组学和蛋白质组学分析。文中展示了胁迫条件下二色补血草叶片转录组学和蛋白质组学分析的实验设计图。对于转录组学,采用了mRNA测序技术。列出了二色补血草叶片在起始点、高强度UV-B照射以及高强度UV-B照射后进行黑暗处理时鉴定出的基因;本文还展示了这些基因在起始点、高强度UV-B照射以及高强度UV-B照射后进行黑暗处理时的不同表达水平。对于蛋白质组学,采用了无凝胶/无标记蛋白质组技术。本文展示了二色补血草叶片在起始点、高强度UV-B照射以及高强度UV-B照射后进行黑暗处理时不同丰度的蛋白质。为了监测胁迫条件下基因的表达水平,进行了定量逆转录聚合酶链反应。本文还展示了用于定量逆转录聚合酶链反应的所选基因的引物序列。