Cosgrove Daniel J, Hepler Nathan K, Wagner Edward R, Durachko Daniel M
Department of Biology, Pennsylvania State University, 350A Frear North Bldg., University Park, PA, 16802, USA.
Methods Mol Biol. 2017;1588:157-165. doi: 10.1007/978-1-4939-6899-2_12.
Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here, we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.
扩展蛋白是一类能使植物细胞壁松弛但缺乏酶活性的蛋白质。在此，我们描述了两种专门用于测量细菌扩展蛋白生物力学活性的实验方案。第一种测定方法基于扩展蛋白对滤纸的弱化作用。第二种测定方法基于植物细胞壁样品蠕变（长期、不可逆伸展）的诱导。
