使用单克隆抗体鉴定牛乳头瘤病毒L1基因产物。

Identification of the bovine papillomavirus L1 gene product using monoclonal antibodies.

作者信息

Cowsert L M, Pilacinski W P, Jenson A B

机构信息

Department of Microbiology, Georgetown University, Washington, DC 20007.

出版信息

Virology. 1988 Aug;165(2):613-5. doi: 10.1016/0042-6822(88)90608-3.

DOI:10.1016/0042-6822(88)90608-3

PMID:2841806

Abstract

Monoclonal antibodies (MoAbs) produced against SDS-disrupted bovine papillomavirus type 1 (BPV-1) were used to identify the product of the L1 open reading frame (ORF) of BPV-1. MoAbs were tested in ELISA with purified BPV-1 major capsid protein, fusion proteins from two constructions of the BPV-1 L1 ORF, and one construction of the L2 ORF. All MoAbs were reactive with purified MCP and both L1 fusion proteins. No MoAbs were identified that were reactive with the L2 fusion protein. Polyclonal antisera raised against SDS-disrupted BPV-1 were immunoreactive with both L1 and the L2 fusion proteins. These data show that the L1 ORF of BPV-1 encodes, at least in part, the MCP of BPV-1. Further, it has been shown that the L1 encodes the papillomavirus (PV) genus-specific epitope, PV broadly cross-reactive epitope, BPV minimally cross-reactive epitope, and a BPV-1 type-specific epitope.

摘要

针对经十二烷基硫酸钠（SDS）处理破坏的1型牛乳头瘤病毒（BPV-1）产生的单克隆抗体（MoAbs），被用于鉴定BPV-1的L1开放阅读框（ORF）的产物。单克隆抗体通过酶联免疫吸附测定（ELISA），用纯化的BPV-1主要衣壳蛋白、两种BPV-1 L1开放阅读框构建体的融合蛋白以及一种L2开放阅读框构建体进行检测。所有单克隆抗体均与纯化的主要衣壳蛋白（MCP）以及两种L1融合蛋白发生反应。未鉴定出与L2融合蛋白发生反应的单克隆抗体。针对经SDS处理破坏的BPV-1产生的多克隆抗血清，与L1和L2融合蛋白均发生免疫反应。这些数据表明，BPV-1的L1开放阅读框至少部分编码BPV-1的主要衣壳蛋白。此外，已表明L1编码乳头瘤病毒（PV）属特异性表位、PV广泛交叉反应性表位、BPV最小交叉反应性表位以及一种BPV-1型特异性表位。