Davis M G, Huang E S
Lineberger Cancer Research Center, Department of Medicine, University of North Carolina, Chapel Hill 27514.
Biotechnol Appl Biochem. 1988 Feb;10(1):6-12.
The human cytomegalovirus immediate-early gene region 1 promoter-enhancer is active in bacteria and in many mammalian cells. Recombinant plasmids containing portions of this DNA can be used to promote the expression of foreign proteins in many cells. In this communication, we report the optimal conditions for transfer of plasmid DNA to cells by electroporation and the transient expression assays which document the activity of different promoter constructions. The observed activity of the human cytomegalovirus promoter is more than 100-fold higher than the activity of the early promoter of SV40.
人巨细胞病毒立即早期基因区域1启动子-增强子在细菌和许多哺乳动物细胞中具有活性。含有该DNA部分片段的重组质粒可用于促进外源蛋白在许多细胞中的表达。在本通讯中，我们报告了通过电穿孔将质粒DNA导入细胞的最佳条件以及记录不同启动子构建体活性的瞬时表达分析。观察到的人巨细胞病毒启动子的活性比SV40早期启动子的活性高100倍以上。
