Iriyoda T M V, Stadtlober N, Lozovoy M A B, Delongui F, Costa N T, Reiche E M V, Dichi I, Simão A N C
1 Department of Rheumatology, Pontifícia Universidade Católica (PUC) do Paraná, Brazil.
2 Post Graduate Program in Experimental Pathology, University of Londrina (UEL), Brazil.
Lupus. 2017 Sep;26(10):1106-1111. doi: 10.1177/0961203317692436. Epub 2017 Mar 22.
The aims of the present study were to evaluate biomarkers of oxidative and nitrosative stress in systemic lupus erythematosus (SLE) patients, in particular products of DNA/RNA oxidative damage and their correlation with disease activity. This study included 188 controls and 203 patients; 153 with inactive SLE (SLEDAI < 6) and 50 with active SLE (SLEDAI ≥ 6) without renal impairment. Oxidative stress was assessed by tert-butyl hydroperoxide-initiated by chemiluminescence, advanced oxidation protein products (AOPP), total radical-trapping antioxidant parameter (TRAP), nitric oxide metabolites (NOx), and DNA/RNA oxidation products. Patients with SLE showed increased oxidative stress, as demonstrated by the augmentation of lipid hydroperoxides ( p < 0.0001) and AOPP ( p < 0.001) and reduced total antioxidant capacity ( p < 0.0001), without differences between patients with active disease and in remission. NOx levels and DNA/RNA oxidation products were inversely and independently associated with disease activity ( p < 0.0001 and p = 0.021, respectively), regardless of BMI and prednisone use. The linear regression analysis showed that about 5% of the SLEDAI score can be explained by the levels of DNA/RNA oxidation products ( r:0.051; p = 0.002) and about 9% of this score by the levels of NOx ( r:0.091; p < 0.0001). This study provides evidence for an inverse association between serum NOx levels and DNA/RNA oxidation products and SLE disease activity, suggesting that oxidative/nitrosative stress markers may be useful in evaluating SLE disease activity and progression of the disease.
本研究的目的是评估系统性红斑狼疮（SLE）患者氧化应激和亚硝化应激的生物标志物，特别是DNA/RNA氧化损伤产物及其与疾病活动的相关性。本研究纳入了188名对照者和203名患者；其中153例为非活动期SLE患者（SLEDAI＜6），50例为无肾功能损害的活动期SLE患者（SLEDAI≥6）。通过叔丁基过氧化氢引发的化学发光、晚期氧化蛋白产物（AOPP）、总自由基捕获抗氧化参数（TRAP）、一氧化氮代谢产物（NOx）以及DNA/RNA氧化产物来评估氧化应激。SLE患者表现出氧化应激增加，脂质过氧化氢增加（p＜0.0001）和AOPP增加（p＜0.001）以及总抗氧化能力降低（p＜0.0001）均证明了这一点，活动期疾病患者与缓解期患者之间无差异。无论BMI和泼尼松的使用情况如何，NOx水平和DNA/RNA氧化产物均与疾病活动呈负相关且独立相关（分别为p＜0.0001和p = 0.021）。线性回归分析表明，SLEDAI评分的约5%可由DNA/RNA氧化产物水平解释（r：0.051；p = 0.002），该评分的约9%可由NOx水平解释（r：0.091；p＜0.0001）。本研究为血清NOx水平和DNA/RNA氧化产物与SLE疾病活动之间的负相关提供了证据，表明氧化/亚硝化应激标志物可能有助于评估SLE疾病活动和疾病进展。
