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家禽眼梳膜中碳酸酐酶和钠钾ATP酶的组织化学显示

Histochemical demonstration of carbonic anhydrase and Na+/K+-ATPase in the pecten oculi of the fowl.

作者信息

Eichhorn M, Flügel C

机构信息

Department of Anatomy, University of Erlangen-Nürnberg, West Germany.

出版信息

Exp Eye Res. 1988 Jul;47(1):147-53. doi: 10.1016/0014-4835(88)90031-0.

DOI:10.1016/0014-4835(88)90031-0
PMID:2842176
Abstract

The distribution of carbonic anhydrase (CA) and Na+/K+-ATPase was studied histochemically in the pecten of the fowl by light and electron microscopy. No Na+/K+-ATPase activity was seen by the method used here. CA staining was seen in the membranes of the apical and basal microvilli of the endothelial cells, while the cytoplasm took no stain. There were no staining differences between the capillaries of the different regions of the pecten. Only the capillaries of the bridge showed no microvilli and no staining. Neither did arterioles and venules which lacked microvilli stain. The functional significance of the association of CA activity and microvilli is not clear.

摘要

通过光学显微镜和电子显微镜对鸡的栉膜进行组织化学研究,观察碳酸酐酶(CA)和钠钾ATP酶的分布情况。采用此处所用方法未观察到钠钾ATP酶活性。在内皮细胞顶端和基底微绒毛的膜上可见CA染色,而细胞质未染色。栉膜不同区域的毛细血管之间染色无差异。只有桥部的毛细血管未显示微绒毛且无染色。缺乏微绒毛的小动脉和小静脉也未染色。CA活性与微绒毛之间关联的功能意义尚不清楚。

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