• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过高通量测序鉴定和表征两种密切相关的 C 和 C 种 Cleome 中的 microRNAs。

Identification and characterization of miRNAs in two closely related C and C species of Cleome by high-throughput sequencing.

机构信息

College of Agriculture, Henan University of Science and Technology, Luoyang, Henan Province, 471003, P. R. China.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, P. R. China.

出版信息

Sci Rep. 2017 Apr 19;7:46552. doi: 10.1038/srep46552.

DOI:10.1038/srep46552
PMID:28422166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5396198/
Abstract

Cleome gynandra and Cleome hassleriana, which are C and C plants, respectively, are two species of Cleome. The close genetic relationship between C. gynandra and C. hassleriana provides advantages for discovering the differences in leaf development and physiological processes between C and C plants. MicroRNAs (miRNAs) are a class of important regulators of various biological processes. In this study, we investigate the differences in the characteristics of miRNAs between C. gynandra and C. hassleriana using high-throughput sequencing technology. In total, 94 and 102 known miRNAs were identified in C. gynandra and C. hassleriana, respectively, of which 3 were specific for C. gynandra and 10 were specific for C. hassleriana. Ninety-one common miRNAs were identified in both species. In addition, 4 novel miRNAs were detected, including three in C. gynandra and three in C. hassleriana. Of these miRNAs, 67 were significantly differentially expressed between these two species and were involved in extensive biological processes, such as glycol-metabolism and photosynthesis. Our study not only provided resources for C. gynandra and C. hassleriana research but also provided useful clues for the understanding of the roles of miRNAs in the alterations of biological processes in leaf tissues during the evolution of the C pathway.

摘要

白花菜属的白花菜和 Hassler 白花菜分别为 C3 和 C4 植物，是白花菜属的两个种。C3 植物白花菜和 C4 植物 Hassler 白花菜之间的密切遗传关系为发现 C3 和 C4 植物在叶片发育和生理过程中的差异提供了优势。microRNAs（miRNAs）是一类重要的调节各种生物过程的调节剂。在这项研究中，我们使用高通量测序技术研究了 C. gynandra 和 C. hassleriana 之间 miRNA 特征的差异。在 C. gynandra 和 C. hassleriana 中分别鉴定出 94 个和 102 个已知的 miRNAs，其中 3 个是 C. gynandra 特有的，10 个是 C. hassleriana 特有的。在这两个物种中鉴定出 91 个共同的 miRNAs。此外，还检测到 4 个新的 miRNAs，包括 3 个在 C. gynandra 中，3 个在 C. hassleriana 中。这些 miRNA 中，有 67 个在这两个物种之间差异显著表达，并参与了广泛的生物学过程，如糖酵解代谢和光合作用。我们的研究不仅为 C. gynandra 和 C. hassleriana 的研究提供了资源，也为理解 miRNA 在 C 途径进化过程中叶片组织中生物过程改变中的作用提供了有用的线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/e0d4b6cada89/srep46552-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/b080d5719df4/srep46552-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/0fa89e11320c/srep46552-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/b35748b69541/srep46552-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/e0d4b6cada89/srep46552-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/b080d5719df4/srep46552-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/0fa89e11320c/srep46552-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/b35748b69541/srep46552-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223c/5396198/e0d4b6cada89/srep46552-f4.jpg

相似文献

1
Identification and characterization of miRNAs in two closely related C and C species of Cleome by high-throughput sequencing.通过高通量测序鉴定和表征两种密切相关的 C 和 C 种 Cleome 中的 microRNAs。
Sci Rep. 2017 Apr 19;7:46552. doi: 10.1038/srep46552.
2
An mRNA blueprint for C4 photosynthesis derived from comparative transcriptomics of closely related C3 and C4 species.基于比较 C3 和 C4 近缘物种的转录组学，得到的 C4 光合作用的 mRNA 蓝图。
Plant Physiol. 2011 Jan;155(1):142-56. doi: 10.1104/pp.110.159442. Epub 2010 Jun 11.
3
A Specific Transcriptome Signature for Guard Cells from the C4 Plant Gynandropsis gynandra.C4植物白花菜保卫细胞的特定转录组特征
Plant Physiol. 2016 Mar;170(3):1345-57. doi: 10.1104/pp.15.01203. Epub 2016 Jan 27.
4
Endoreduplication is not involved in bundle-sheath formation in the C4 species Cleome gynandra.内复制不参与C4植物白花菜束鞘的形成。
J Exp Bot. 2014 Jul;65(13):3557-66. doi: 10.1093/jxb/ert350. Epub 2013 Nov 12.
5
Photosynthetic, hydraulic and biomass properties in closely related C3 and C4 species.在密切相关的 C3 和 C4 物种中，光合、水力和生物量特性。
Physiol Plant. 2015 Mar;153(3):454-66. doi: 10.1111/ppl.12240. Epub 2014 Jul 29.
6
Post-transcriptional regulation of photosynthetic genes is a key driver of C4 leaf ontogeny.光合基因的转录后调控是C4叶片个体发育的关键驱动因素。
J Exp Bot. 2017 Jan;68(2):137-146. doi: 10.1093/jxb/erw386. Epub 2016 Oct 18.
7
Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C₄) and Cleome spinosa (C₃) under drought stress.干旱胁迫下 C₄ 模式植物白花菜（Cleome gynandra）和 C₃ 模式植物刺山柑（Cleome spinosa）活性氧形成和抗氧化酶的比较。
Plant Sci. 2012 Jan;182:59-70. doi: 10.1016/j.plantsci.2011.03.015. Epub 2011 Apr 6.
8
Diversity in forms of C4 in the genus Cleome (Cleomaceae).Cleome 属（山柑科）中 C4 形式的多样性。
Ann Bot. 2011 Feb;107(2):269-83. doi: 10.1093/aob/mcq239. Epub 2010 Dec 8.
9
Physiological, anatomical and biochemical characterisation of photosynthetic types in genus Cleome (Cleomaceae).醉蝶花属（白花菜科）光合类型的生理、解剖及生化特征
Funct Plant Biol. 2007 May;34(4):247-267. doi: 10.1071/FP06287.
10
Whole-Genome Duplication Facilitated the Evolution of C4 Photosynthesis in Gynandropsis gynandra.全基因组复制促进了 Gynandropsis gynandra 中 C4 光合作用的进化。
Mol Biol Evol. 2021 Oct 27;38(11):4715-4731. doi: 10.1093/molbev/msab200.

引用本文的文献

1
Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.南极绿藻莱茵衣藻ICE-L中三种α-碳酸酐酶在不同光照胁迫处理下的分离与表达分析
Mol Biotechnol. 2019 Mar;61(3):200-208. doi: 10.1007/s12033-018-00152-4.

本文引用的文献

1
The evolution of C photosynthesis.C4光合作用的进化。
New Phytol. 2004 Feb;161(2):341-370. doi: 10.1111/j.1469-8137.2004.00974.x.
2
High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds.基于高通量测序的棉花发育种子中表达的微小RNA全基因组鉴定。
Sci China Life Sci. 2015 Aug;58(8):778-86. doi: 10.1007/s11427-015-4877-5. Epub 2015 Jun 27.
3
Plant miRNAs: biogenesis, organization and origins.植物微小RNA：生物合成、组织及起源
Funct Integr Genomics. 2015 Sep;15(5):523-31. doi: 10.1007/s10142-015-0451-2. Epub 2015 Jun 26.
4
Genome-wide comparison of microRNAs and their targeted transcripts among leaf, flower and fruit of sweet orange.甜橙叶片、花朵和果实中微小RNA及其靶向转录本的全基因组比较。
BMC Genomics. 2014 Aug 20;15(1):695. doi: 10.1186/1471-2164-15-695.
5
Comparative transcriptome atlases reveal altered gene expression modules between two Cleomaceae C3 and C4 plant species.比较转录组图谱揭示了两种十字花科C3和C4植物物种之间基因表达模块的变化。
Plant Cell. 2014 Aug;26(8):3243-60. doi: 10.1105/tpc.114.123752. Epub 2014 Aug 8.
6
Ethylene-insensitive3 is a senescence-associated gene that accelerates age-dependent leaf senescence by directly repressing miR164 transcription in Arabidopsis.乙烯不敏感3是一种衰老相关基因，它通过直接抑制拟南芥中miR164的转录来加速依赖年龄的叶片衰老。
Plant Cell. 2013 Sep;25(9):3311-28. doi: 10.1105/tpc.113.113340. Epub 2013 Sep 24.
7
Biogenesis, turnover, and mode of action of plant microRNAs.植物 microRNAs 的生物发生、周转和作用模式。
Plant Cell. 2013 Jul;25(7):2383-99. doi: 10.1105/tpc.113.113159. Epub 2013 Jul 23.
8
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching.miRNA OsmiR397 的过表达通过增加粒长和促进穗分枝提高水稻产量。
Nat Biotechnol. 2013 Sep;31(9):848-52. doi: 10.1038/nbt.2646. Epub 2013 Jul 21.
9
Identification of microRNA 395a in 24-epibrassinolide-regulated root growth of Arabidopsis thaliana using microRNA arrays.利用微RNA芯片鉴定24-表油菜素内酯调控拟南芥根生长过程中的微RNA 395a
Int J Mol Sci. 2013 Jul 9;14(7):14270-86. doi: 10.3390/ijms140714270.
10
Identification and expression analysis of microRNAs at the grain filling stage in rice(Oryza sativa L.)via deep sequencing.通过深度测序鉴定和表达分析水稻灌浆期的 microRNAs。
PLoS One. 2013;8(3):e57863. doi: 10.1371/journal.pone.0057863. Epub 2013 Mar 1.