• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

微小RNA-22通过Sp1负反馈环抑制结肠癌细胞的生长、迁移和侵袭。

MicroRNA-22 suppresses the growth, migration and invasion of colorectal cancer cells through a Sp1 negative feedback loop.

作者信息

Xia Shu-Sen, Zhang Guang-Jun, Liu Zuo-Liang, Tian Hong-Peng, He Yi, Meng Chang-Yuan, Li Li-Fa, Wang Zi-Wei, Zhou Tong

机构信息

The Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

The Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.

出版信息

Oncotarget. 2017 May 30;8(22):36266-36278. doi: 10.18632/oncotarget.16742.

DOI:10.18632/oncotarget.16742
PMID:28422727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5482653/
Abstract

MicroRNAs have recently emerged as regulators of many biological processes including cell proliferation, development and differentiation. This study identified that miR-22 was statistically decreased in colorectal cancer clinical specimens and highly metastatic cell lines. Moreover, low miR-22 expression was associated with tumor metastasis, advanced clinical stage and relapse. Consistent with clinical observations, miR-22 significantly suppressed the ability of colorectal cancer cells to growth and metastasize in vitro and in vivo. Sp1 was validated as a target of miR-22, and ectopic expression of Sp1 compromised the inhibitory effects of miR-22. In addition, Sp1 repressed miR-22 transcription by binding to the miR-22 promoter, hence forming a negative feedback loop. Further study has shown that miR-22 suppresses the activity of PTEN/AKT pathway by Sp1. Our present results implicate the newly indentified miR-22/Sp1/PTEN/AKT axis might represent a potential therapeutic target for colorectal cancer.

摘要

微小RNA最近已成为包括细胞增殖、发育和分化在内的许多生物学过程的调节因子。本研究发现，在结直肠癌临床标本和高转移细胞系中，miR-22在统计学上有所降低。此外，低miR-22表达与肿瘤转移、临床晚期和复发相关。与临床观察结果一致，miR-22在体外和体内均显著抑制结直肠癌细胞的生长和转移能力。Sp1被验证为miR-22的靶标，Sp1的异位表达削弱了miR-22的抑制作用。此外，Sp1通过与miR-22启动子结合来抑制miR-22转录，从而形成负反馈回路。进一步研究表明，miR-22通过Sp1抑制PTEN/AKT通路的活性。我们目前的结果表明，新发现的miR-22/Sp1/PTEN/AKT轴可能代表结直肠癌的一个潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/df715e02ad35/oncotarget-08-36266-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/b233aca26e8b/oncotarget-08-36266-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/c3cac0cb1b46/oncotarget-08-36266-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/82f1937a68c5/oncotarget-08-36266-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/9804b49ae483/oncotarget-08-36266-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/549fa9e80dc1/oncotarget-08-36266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/d918b442006d/oncotarget-08-36266-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/a852a879e0ca/oncotarget-08-36266-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/df715e02ad35/oncotarget-08-36266-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/b233aca26e8b/oncotarget-08-36266-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/c3cac0cb1b46/oncotarget-08-36266-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/82f1937a68c5/oncotarget-08-36266-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/9804b49ae483/oncotarget-08-36266-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/549fa9e80dc1/oncotarget-08-36266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/d918b442006d/oncotarget-08-36266-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/a852a879e0ca/oncotarget-08-36266-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe5a/5482653/df715e02ad35/oncotarget-08-36266-g008.jpg

相似文献

1
MicroRNA-22 suppresses the growth, migration and invasion of colorectal cancer cells through a Sp1 negative feedback loop.微小RNA-22通过Sp1负反馈环抑制结肠癌细胞的生长、迁移和侵袭。
Oncotarget. 2017 May 30;8(22):36266-36278. doi: 10.18632/oncotarget.16742.
2
STAT1 Inhibits MiR-181a Expression to Suppress Colorectal Cancer Cell Proliferation Through PTEN/Akt.信号转导及转录激活因子1（STAT1）通过磷酸酶和张力蛋白同源物（PTEN）/蛋白激酶B（Akt）抑制微小RNA-181a（miR-181a）表达，从而抑制结肠癌细胞增殖。
J Cell Biochem. 2017 Oct;118(10):3435-3443. doi: 10.1002/jcb.26000. Epub 2017 Jun 7.
3
MiR-21 regulates biological behavior through the PTEN/PI-3 K/Akt signaling pathway in human colorectal cancer cells.miR-21 通过 PTEN/PI-3 K/Akt 信号通路调节人结直肠癌细胞的生物学行为。
Int J Oncol. 2013 Jan;42(1):219-28. doi: 10.3892/ijo.2012.1707. Epub 2012 Nov 20.
4
MiR-650 represses high-risk non-metastatic colorectal cancer progression via inhibition of AKT2/GSK3β/E-cadherin pathway.微小RNA-650通过抑制AKT2/GSK3β/E-钙黏蛋白通路抑制高危非转移性结直肠癌进展。
Oncotarget. 2017 Jul 25;8(30):49534-49547. doi: 10.18632/oncotarget.17743.
5
miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1.微小RNA-29b通过靶向Sp1，经由PTEN-AKT信号通路抑制舌鳞状细胞癌的增殖、迁移和侵袭。
Oral Oncol. 2014 Nov;50(11):1062-71. doi: 10.1016/j.oraloncology.2014.07.010. Epub 2014 Aug 7.
6
miR-5188 augments glioma growth, migration and invasion through an SP1-modulated FOXO1-PI3K/AKT-c-JUN-positive feedback circuit.miR-5188 通过 SP1 调节的 FOXO1-PI3K/AKT-c-JUN 正反馈回路促进胶质瘤的生长、迁移和侵袭。
J Cell Mol Med. 2020 Oct;24(20):11800-11813. doi: 10.1111/jcmm.15794. Epub 2020 Sep 9.
7
MicroRNA‑760 inhibits cell proliferation and invasion of colorectal cancer by targeting the SP1‑mediated PTEN/AKT signalling pathway.微小 RNA-760 通过靶向 SP1 介导的 PTEN/AKT 信号通路抑制结直肠癌细胞增殖和侵袭。
Mol Med Rep. 2017 Dec;16(6):9692-9700. doi: 10.3892/mmr.2017.7814. Epub 2017 Oct 17.
8
Regulation of colorectal carcinoma stemness, growth, and metastasis by an miR-200c-Sox2-negative feedback loop mechanism.miR-200c-Sox2 负反馈环路机制调控结直肠癌干细胞干性、生长和转移。
Clin Cancer Res. 2014 May 15;20(10):2631-42. doi: 10.1158/1078-0432.CCR-13-2348. Epub 2014 Mar 21.
9
A regulatory loop involving miR-22, Sp1, and c-Myc modulates CD147 expression in breast cancer invasion and metastasis.一个包含 miR-22、Sp1 和 c-Myc 的调控环调节乳腺癌侵袭和转移中的 CD147 表达。
Cancer Res. 2014 Jul 15;74(14):3764-78. doi: 10.1158/0008-5472.CAN-13-3555. Epub 2014 Jun 6.
10
miR-375 inhibits the invasion and metastasis of colorectal cancer via targeting SP1 and regulating EMT-associated genes.miR-375 通过靶向 SP1 并调节 EMT 相关基因抑制结直肠癌的侵袭和转移。
Oncol Rep. 2016 Jul;36(1):487-93. doi: 10.3892/or.2016.4834. Epub 2016 May 24.

引用本文的文献

1
Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.乳源细胞外囊泡与微小RNA：肠道稳态的潜在调节因子
FASEB J. 2025 Aug 31;39(16):e70947. doi: 10.1096/fj.202501630R.
2
Inflammasomes in Intestinal Disease: Mechanisms of Activation and Therapeutic Strategies.肠道疾病中的炎性小体：激活机制与治疗策略
Int J Mol Sci. 2024 Dec 4;25(23):13058. doi: 10.3390/ijms252313058.
3
Retraction: Myeloid ecotropic viral integration site 1 inhibits cell proliferation, invasion or migration in human gastric cancer.

本文引用的文献

1
miR-375 inhibits the invasion and metastasis of colorectal cancer via targeting SP1 and regulating EMT-associated genes.miR-375 通过靶向 SP1 并调节 EMT 相关基因抑制结直肠癌的侵袭和转移。
Oncol Rep. 2016 Jul;36(1):487-93. doi: 10.3892/or.2016.4834. Epub 2016 May 24.
2
miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemia.miR-22 在急性髓系白血病中具有强大的抗肿瘤作用和治疗潜力。
Nat Commun. 2016 Apr 26;7:11452. doi: 10.1038/ncomms11452.
3
MicroRNAs in colorectal carcinoma--from pathogenesis to therapy.
撤稿声明：髓系嗜亲性病毒整合位点1抑制人胃癌细胞的增殖、侵袭和迁移。
Oncotarget. 2024 Sep 17;15:634. doi: 10.18632/oncotarget.28649.
4
Diagnostic and Prognostic Value of miR-451 Expression in Colorectal Cancer: A Meta-Analysis.miR-451 在结直肠癌中的诊断和预后价值：一项荟萃分析。
Asian Pac J Cancer Prev. 2024 Jun 1;25(6):1903-1910. doi: 10.31557/APJCP.2024.25.6.1903.
5
Beneficial Effect of Olive Oil and Its Derivates: Focus on Hematological Neoplasm.橄榄油及其衍生物的有益作用：聚焦血液肿瘤
Life (Basel). 2024 Apr 30;14(5):583. doi: 10.3390/life14050583.
6
AP4 induces JNK1 and a miR-22-3p/FOSL1 feed-forward loop to activate AP-1 and promote colorectal cancer metastasis.AP4诱导JNK1以及一个miR-22-3p/FOSL1前馈环以激活AP-1并促进结直肠癌转移。
Cancer Commun (Lond). 2024 Mar;44(3):433-437. doi: 10.1002/cac2.12514. Epub 2024 Jan 15.
7
miR-22 Suppresses EMT by Mediating Metabolic Reprogramming in Colorectal Cancer through Targeting MYC-Associated Factor X.miR-22 通过靶向 MYC 相关因子 X 调控代谢重编程抑制结直肠癌中的 EMT
Dis Markers. 2022 Aug 25;2022:7843565. doi: 10.1155/2022/7843565. eCollection 2022.
8
miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in colorectal cancer.miR-320a/SP1负向相互作用促进结直肠癌的细胞生长和侵袭。
Cancer Cell Int. 2021 Mar 17;21(1):175. doi: 10.1186/s12935-021-01874-3.
9
Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review.与结直肠癌预后相关的组织微小RNA：一项系统评价
Mol Biol Rep. 2021 Feb;48(2):1853-1867. doi: 10.1007/s11033-020-06075-1. Epub 2021 Feb 17.
10
The therapeutic potential of exosomal miR-22 for cervical cancer radiotherapy.外泌体 miR-22 在宫颈癌放疗中的治疗潜力。
Cancer Biol Ther. 2020 Dec 1;21(12):1128-1135. doi: 10.1080/15384047.2020.1838031. Epub 2020 Nov 16.
结直肠癌中的微小RNA——从发病机制到治疗
J Exp Clin Cancer Res. 2016 Mar 10;35:43. doi: 10.1186/s13046-016-0320-4.
4
Sp1 and Sp3 Are the Transcription Activators of Human ek1 Promoter in TSA-Treated Human Colon Carcinoma Cells.Sp1和Sp3是曲古抑菌素A处理的人结肠癌细胞中人类ek1启动子的转录激活因子。
PLoS One. 2016 Jan 25;11(1):e0147886. doi: 10.1371/journal.pone.0147886. eCollection 2016.
5
Transcription Factor Sp1 Promotes the Expression of Porcine ROCK1 Gene.转录因子Sp1促进猪ROCK1基因的表达。
Int J Mol Sci. 2016 Jan 15;17(1):112. doi: 10.3390/ijms17010112.
6
Sp1-mediated ectopic expression of T-cell lymphoma invasion and metastasis 2 in hepatocellular carcinoma.Sp1介导的T细胞淋巴瘤侵袭与转移因子2在肝细胞癌中的异位表达
Cancer Med. 2016 Mar;5(3):465-77. doi: 10.1002/cam4.611. Epub 2016 Jan 14.
7
Mechanisms of regulation of mature miRNAs.成熟微RNA的调控机制。
Biochem Soc Trans. 2015 Dec;43(6):1208-14. doi: 10.1042/BST20150157.
8
MicroRNA-22 inhibits tumor growth and metastasis in gastric cancer by directly targeting MMP14 and Snail.微小RNA-22通过直接靶向基质金属蛋白酶14（MMP14）和蜗牛蛋白（Snail）抑制胃癌的肿瘤生长和转移。
Cell Death Dis. 2015 Nov 26;6(11):e2000. doi: 10.1038/cddis.2015.297.
9
PTEN Overexpression Cooperates With Lithium to Reduce the Malignancy and to Increase Cell Death by Apoptosis via PI3K/Akt Suppression in Colorectal Cancer Cells.PTEN过表达与锂协同作用，通过抑制PI3K/Akt来降低结直肠癌细胞的恶性程度并增加细胞凋亡导致的细胞死亡。
J Cell Biochem. 2016 Feb;117(2):458-69. doi: 10.1002/jcb.25294.
10
SP1-mediated microRNA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1.SP1介导的微小RNA-520d-5p通过靶向CTHRC1抑制结直肠癌的肿瘤生长和转移。
Am J Cancer Res. 2015 Mar 15;5(4):1447-59. eCollection 2015.