评价 PDGF-BB 与不同载体联合在大鼠骨外科缺损骨再生中的成骨诱导作用。
Evaluation of the Osteoinductive Effect of PDGF-BB Associated With Different Carriers in Bone Regeneration in Bone Surgical Defects in Rats.
机构信息
*Departament of Surgery and Integrated Clinic, Araçatuba Dental School. Araçatuba, SP, Brazil. †Assistant Professor, Departament of Anatomy, Araçatuba Dental School, Araçatuba, SP, Brazil. ‡Adjunct Professor, Department of Surgery and Integrated Clinic, Aracatuba Dental School, Araçatuba, SP, Brazil. §Private Practice, Department of Surgery and Integrated Clinic, Aracatuba Dental School, Araçatuba, SP, Brazil.
出版信息
Implant Dent. 2017 Aug;26(4):559-566. doi: 10.1097/ID.0000000000000580.
PURPOSE
Extensive bone defects in maxillofacial region can be corrected with autograft. However the disadvantages of this type of therapy lead to the search for new bone substitutes. Thus, we evaluated the biological behavior and osteoinductive platelet-derived growth factor type BB (PDGF-BB) associated with different carriers, by histological analysis and immunohistochemical histometric critical defects performed in rat calvaria.
MATERIALS AND METHODS
Critical defects were created with 5-mm diameter calvaria of rats. Each defect was randomly divided into 8 experimental groups, evaluated at 15 and 30 postoperative days for histomorphometry and immunohistochemistry.
RESULTS
The results showed new bone formation in all groups, independent of postoperative time. At 30 days, the beta-tricalcium phosphate matrix (TCP) group just did not differ from bone matrix mineralized bovine (BIO) group in the new bone formation (P = 0.1403). In none of the groups' analyzed biomaterials, growth factor stimulated increase in bone formation (P > 0.05).
CONCLUSION
With the methodology used, the growth factor associated with the tested biomaterials did not induce new bone formation.
目的
大量的颌面骨缺损可以通过自体骨移植来矫正。然而，这种治疗方法的缺点导致了对新型骨替代物的探索。因此，我们通过对大鼠颅骨临界缺损进行组织学分析和免疫组织化学组织计量学评估，研究了不同载体与血小板衍生生长因子 BB（PDGF-BB）的生物行为和骨诱导作用。
材料和方法
通过大鼠 5mm 直径颅骨创建临界缺损。每个缺损随机分为 8 个实验组，在术后 15 天和 30 天进行组织形态计量学和免疫组织化学评估。
结果
结果显示所有组均有新骨形成，与术后时间无关。在 30 天，β-磷酸三钙（TCP）组与牛骨基质矿化（BIO）组的新骨形成没有差异（P=0.1403）。在分析的生物材料中，没有一种生长因子刺激骨形成增加（P>0.05）。
结论
采用该方法，生长因子与测试的生物材料结合并未诱导新骨形成。
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