安卡福司特对糖尿病大鼠皮肤愈合的影响。

Effects of Ankaferd BloodStopper on dermal healing in diabetic rats.

机构信息

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Marmara University, İstanbul, Turkey.

Experimental Research Unit, Faculty of Engineering and Natural Sciences, Üsküdar University, İstanbul, Turkey.

出版信息

Turk J Med Sci. 2017 Apr 18;47(2):675-680. doi: 10.3906/sag-1604-145.

DOI:10.3906/sag-1604-145

PMID:28425265

Abstract

BACKGROUND/AIM: Diabetes mellitus inhibits wound-induced angiogenesis, impairs the wound healing process, and leads to the development of chronic wounds. Ankaferd BloodStopper (ABS) is a new and promising local haemostatic agent. Although the mechanism of ABS-mediated haemostasis is well established, little is known about the associated histological and biochemical tissue reactions. The aim of this study was to evaluate the effects of this new-generation local haemostatic agent on short-term soft-tissue healing in streptozotocin (STZ)-treated rats.

MATERIALS AND METHODS

The 24 Wistar albino rats used in this study were divided into STZ-treated (STZ, n = 12) and nontreated groups (control, n = 12). Four days prior to surgery, rats in the STZ group were subcutaneously administered 60 mg/kg STZ intraperitoneally, while rats in the control group were administered 1 mL saline/kg. An incision was made in the dorsal dermal tissue of all rats, and either ABS or no haemostatic agent (NHAA) was applied to the wound before suturing. All of the rats were euthanised on postoperative day 4. Blood and skin samples were evaluated biochemically and histologically.

RESULTS

The results showed that STZ treatment impaired soft-tissue healing, assessed by measuring glutathione and lipid peroxidation levels. Moreover, while good histological results were obtained in the control group treated with ABS, there were fewer benefits in the STZ-treated group.

CONCLUSION

ABS's benefits in the control group seemed to lose their effectiveness under STZ medication.

摘要

背景/目的：糖尿病抑制伤口诱导的血管生成，损害伤口愈合过程，导致慢性伤口的形成。安可福止血粉（ABS）是一种新型且有前途的局部止血剂。尽管 ABS 介导止血的机制已经得到很好的阐明，但关于其相关的组织学和生化反应知之甚少。本研究旨在评估这种新一代局部止血剂对链脲佐菌素（STZ）处理的大鼠短期软组织愈合的影响。

材料和方法

本研究使用了 24 只 Wistar 白化大鼠，分为 STZ 处理组（STZ，n = 12）和未处理组（对照组，n = 12）。在手术前 4 天，STZ 组大鼠经腹腔内给予 60mg/kg STZ，对照组大鼠给予 1ml/kg 生理盐水。所有大鼠的背部真皮组织均做切口，缝合前应用 ABS 或无止血剂（NHAA）处理伤口。所有大鼠均在术后第 4 天处死。对血液和皮肤样本进行生化和组织学评估。