Ahmad M, Ansari M N, Alam A, Khan T H
Department of Pharmacology, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
Pak J Biol Sci. 2013 Oct 15;16(20):1086-94. doi: 10.3923/pjbs.2013.1086.1094.
Diabetic patients wound healing is slower than the healthy individuals. Three citrus peel extracts; Lemon (Citrus limon), Grapes fruits (Citrus paradise) and Orange (Citrus sinensis) promote wound healing in experimental animals. This study investigated the effect of oral treatment with citrus peel extracts on wound repair of the skin of diabetic rats. The extracts were estimated for vitamin C and total carotenoid contents prior to animal study. Diabetes mellitus was induced in rats by intraperitoneal injection of a single dose of streptozotocin (STZ, 75 mg kg(-1) b.wt.). One week after diabetes induction, full thickness excision wounds were made in hyperglycemic rats and were divided groups, each containing 6 rats. The different test group animals were treated with different citrus peel extract orally at the dose of 400 mg kg(-1) body weight daily for 12 days. The blood glucose, body weight and rate of wound closure of each rat were measured every 3rd day during the experimental period. At the end of experiment, granular tissues of wounds were removed and estimated for hydroxylproline and total protein content. The results showed significant reduction in blood glucose and time to wound closure. Tissue growth and collagen synthesis were significantly higher as determined by total protein and hydroxyl proline content. From our experimental data, we propose that oral administration of citrus peel extracts has a therapeutic potential in the treatment of chronic wounds in diabetes.
糖尿病患者伤口愈合比健康个体慢。三种柑橘皮提取物，柠檬（Citrus limon）、葡萄柚（Citrus paradise）和橙子（Citrus sinensis）可促进实验动物的伤口愈合。本研究调查了口服柑橘皮提取物对糖尿病大鼠皮肤伤口修复的影响。在动物研究之前，对提取物的维生素C和总类胡萝卜素含量进行了测定。通过腹腔注射单剂量链脲佐菌素（STZ，75 mg kg(-1)体重）诱导大鼠患糖尿病。糖尿病诱导一周后，在高血糖大鼠身上制作全层切除伤口，并将其分组，每组包含6只大鼠。不同试验组动物每天按400 mg kg(-1)体重的剂量口服不同的柑橘皮提取物，持续12天。在实验期间，每3天测量一次每只大鼠的血糖、体重和伤口闭合率。实验结束时，取出伤口的肉芽组织并测定羟脯氨酸和总蛋白含量。结果显示血糖显著降低，伤口愈合时间缩短。通过总蛋白和羟脯氨酸含量测定，组织生长和胶原蛋白合成显著增加。根据我们的实验数据，我们提出口服柑橘皮提取物在治疗糖尿病慢性伤口方面具有治疗潜力。
