Teshima S, Shimosato Y, Hirohashi S, Tome Y, Hayashi I, Kanazawa H, Kakizoe T
Pathology Division, National Cancer Center Research Institute, National Cancer Center Hospital, Tokyo, Japan.
Lab Invest. 1988 Sep;59(3):328-36.
Four new human tumor cell lines, NCC-EC-1, NCC-EC-2, NCC-EC-3, and NCC-IT, were derived from different germ cell tumors established in vitro. On the basis of morphologic studies of cell cultures and nude mice xenografts, it was concluded that NCC-EC-1, and NCC-EC-2 cell lines are equivalent to developmentally nullipotent embryonal carcinoma; cell line NCC-EC-3 showed trophoblastic differentiation, whereas the NCC-IT cell line was composed of developmentally pluripotent cells capable of somatic and extraembryonic differentiation. Nude mouse-xenografts of NCC-IT contained foci of embryonal carcinoma, yolk sac tumor, immature somatic tissues, and trophoblastic giant cells indicating that this cell line is indeed developmentally pluripotent. We conclude that human embryonal carcinoma cell lines may be developmentally nullipotent, show restricted capacity for differentiation, or be developmentally pluripotent.
四种新的人类肿瘤细胞系，即NCC-EC-1、NCC-EC-2、NCC-EC-3和NCC-IT，源自体外建立的不同生殖细胞肿瘤。基于细胞培养和裸鼠异种移植的形态学研究，得出结论：NCC-EC-1和NCC-EC-2细胞系等同于发育上无分化潜能的胚胎癌；NCC-EC-3细胞系显示出滋养层分化，而NCC-IT细胞系由能够进行体细胞和胚外分化的发育上多能的细胞组成。NCC-IT的裸鼠异种移植包含胚胎癌、卵黄囊瘤、未成熟体细胞组织和滋养层巨细胞灶，表明该细胞系确实具有发育上的多能性。我们得出结论，人类胚胎癌细胞系可能在发育上无分化潜能、显示出有限的分化能力或具有发育上的多能性。
